Experimental study of the production of bioethanol from wheat and corn fermentation
Tutor / director / avaluadorMorrison, Jason
Tipus de documentProjecte/Treball Final de Carrera
Condicions d'accésAccés restringit per acord de confidencialitat
The project consists on the experimental study of the ethanol fermentation from wheat and corn. The purpose of this project is to analyse the cell growth of the yeast Sac-charomices Cerevisiae, which is used to ferment starch, and analyse the ethanol produc-tion from this process. The starch has to be gelatinized and liquefied by means of the en-zymes α-amylase and amyloglucosidase due to the S. Cerevisiae’s lack of lack of amylo-lytic enzymes. Cell growth curves for YPD, 2% and 1% corn, and 1% winter wheat were performed in the laboratory. Samples were taken at intervals of 1, 1, 1, 2, 2, 2 and 3h to measure OD and ethanol content. The results show that YPD media produces 6.34 g/L with a yield of 0.69. Consequently, 2% corn produces 5.46 g/L with a yield of 0.53. Sub-sequently, 1% corn produces 3.34 g/L with a yield of 0.65; and eventually, 1% winter wheat produces 2.69 g/L with a yield of 0.52. It can be observed that YPD media is the more efficient because it has the largest ethanol production and yield. Two percent corn produces the second largest amount of ethanol, although the yield is low. One percent corn produces a good amount of ethanol, but the yield is extremely high. Finally, 1% winter wheat has the lowest ethanol production and yield.
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