Browsing by Author "Gualda Manzano, Emilio José"Més informacióMés informació

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    • 3D volume rendering of invertebrates using light-sheet fluorescence microscopy 

      Gualda Manzano, Emilio José; Moreno, Nuno (2015-08)
      Article
      Open Access
      Light-Sheet Fluorescence Microscopy (LSFM) has recently emerged as the technique of choice for obtaining high quality 3D images of whole organisms with low photo-damage and fast acquisition rates. Unlike conventional ...

    • 3D Volume rendering of invertebrates using light-sheet fluorescence microscopy 

      Gualda Manzano, Emilio José (2022-03-22)
      Article
      Open Access
      <jats:p>Light-Sheet Fluorescence Microscopy has recently emerged as the technique of choice for obtaining high quality three-dimensional (3D) images of whole organisms, with low photo-damage and fast acquisition rates. ...

    • 3D-3-culture: A tool to unveil macrophage plasticity in the tumour microenvironment 

      Rebelo, Sofia P.; Martins, Tatiana R.; Estrada, Marta F.; Loza Álvarez, Pablo; Harrer, Nathalie; Alves, Paula M.; Cabeçadas, Jose; Gualda Manzano, Emilio José; Sommergruber, Wolfgang; Brito, Catarina (2018-05)
      Article
      Open Access
      The tumour microenvironment (TME) shapes disease progression and influences therapeutic response.Most aggressive solid tumours have high levels of myeloid cell infiltration, namely tumour associatedmacrophages (TAM). ...

    • A computational diffusion model to study antibody transport within reconstructed tumor microenvironments 

      Gualda Manzano, Emilio José; Marsal, Maria; Isidro, Inês; Almeida, Jaime (2020-12)
      Article
      Open Access
      Antibodies revolutionized cancer treatment over the past decades. Despite their successfully application, there are still challenges to overcome to improve efficacy, such as the heterogeneous distribution of antibodies ...

    • A novel culture method that sustains ERa signaling in human breast cancer tissue microstructures 

      Gualda Manzano, Emilio José; Furtado de Oliveira e Silva, Fernanda; Loza Álvarez, Pablo; Cartaxo, Ana Luisa; Estrada, Marta F.; Domenici, Giacomo; Sflomos, George; Brisken, Cathrin; Brito, Catarian (2020-12)
      Article
      Open Access
      Breast cancer (BC) is the most commonly diagnosedcancer among women worldwide [1]. It is a heteroge-neous disease with distinct biological features and clin-ical outcomes. Almost 75% of diagnosed BC expressestrogen ...

    • Adaptive optics multiphoton microscopy 

      Artal, P; Gualda Manzano, Emilio José; Bueno García, Juan Manuel (2010)
      Conference report
      Open Access
      We have developed an adaptive optics multiphoton microscope. The multiphoton imaging system combines an ultrafast high-power laser, a scanning unit, a motorized Z-scan device and a photon-counting detector. The adaptive ...

    • Adaptive optics multiphoton microscopy to study ex vivo ocular tissues 

      Bueno García, Juan Manuel; Gualda Manzano, Emilio José; Artal Soriano, Pablo (2010)
      Article
      Restricted access - publisher's policy
      We develop an adaptive optics (AO) multiphoton microscope by incorporating a deformable mirror and a Hartmann-Shack wavefront sensor. The AO module operating in closed-loop is used to correct for the aberrations of the ...

    • Analysis of aberrations effect in nonlinear processes for femtosecond laser pulses 

      Romero Altimis, C.; Gualda Manzano, Emilio José; Artal Soriano, Pablo; Bueno García, Juan Manuel; Borrego Varillas, Rocío; Alonso Fernández, Benjamín; Méndez Valverde, Cruz; Rodríguez Vázquez de Aldana, Javier; Roso Franco, Luis (Optical Society of American (OSA), 2009)
      Conference report
      Restricted access - publisher's policy
      A method for the analysis and control of femtosecond pulse wavefronts generated in non-linear crystals by three-wave mixing processes is presented. The possibility to improve the efficiency of these processes is discussed.

    • Analysis of corneal stroma organization with wavefront optimized nonlinear microscopy 

      Bueno García, Juan Manuel; Gualda Manzano, Emilio José; Artal Soriano, Pablo (2011-06)
      Article
      Open Access
      To investigate the organization of stromal collagen in healthy ex vivo corneas of different species from second harmonic generation (SHG) microscopy images

    • Analysis of intracellular protein dynamics in living zebrafish embryos using light-sheet fluorescence single-molecule microscopy 

      Bernardello, Matteo; Gualda Manzano, Emilio José; Castro Olvera, Gustavo; Loza Álvarez, Pablo (2021-10-01)
      Article
      Open Access
      Single-molecule microscopy techniques have emerged as useful tools to image individual molecules and analyze their dynamics inside cells, but their application has mostly been restricted to cell cultures. Here, a light-sheet ...

    • Analysis of the chicken retina with an adaptive optics multiphoton microscope 

      Gualda Manzano, Emilio José; Artal Soriano, Pablo; Bueno García, Juan Manuel; Giakoumaki, Anastasia; Schaeffel, Frank (2011-06-01)
      Article
      Restricted access - publisher's policy
      The structure and organization of the chicken retina has been investigated with an adaptive optics multiphoton imaging microscope in a backward configuration. Non-stained flat-mounted retinal tissues were imaged at ...

    • Autofluorescence of stingray skeletal cartilage: hyperspectral imaging as a tool for histological characterization 

      de Juan, Ana; Dean, Mason; Gualda Manzano, Emilio José; Marsal, Maria; Loza Álvarez, Pablo; Chaumel, Júlia; Gómez Sánchez, Adrián; Blumer, Michael (Springer Science and Business Media LLC, 2021-12)
      Audiovisual
      Open Access
      Tessellated cartilage is a distinctive composite tissue forming the bulk of the skeleton of cartilaginous fishes (e.g. sharks and rays), built from unmineralized cartilage covered at the surface by a thin layer of mineralized ...

    • BIOLOGIA GENERAL: BiE Plantas: Módulo VI 

      Gualda Manzano, Emilio José (Universitat Politècnica de Catalunya, 2023-07-11)
      Lecture notes
      Open Access

    • Compensation of third-order dispersion in a 100Gb/s single channel system with in-line fibre Bragg gratings 

      Gualda Manzano, Emilio José; Gómez Pavón, Luz del Carmen; Torres, Juan P. (2005-06-15)
      Article
      Open Access
      This paper presents a scheme to enhance the performance of an ultrahigh capacity(100 Gb/s) long haul transmission system that makes use of chirped fibre Bragggratings (FBG) for dispersion slope compensation. It is shown ...

    • Deficiency of the ywhaz gene, involved in neurodevelopmental disorders, alters brain activity and behaviour in zebrafish 

      Antón Galindo, Ester; Dalla Vecchia, Luisa Felix; Gómez Orlandi, Javier; Castro Olvera, Gustavo; Guasch Piqueras, Marc; Arenas Sola, Concepción; García Fernandez, Jordi; Gualda Manzano, Emilio José; Herrera Úbeda, Carlos; Aguado Tomas, Fernando; Loza Álvarez, Pablo; Cormand, Bru; Norton, William H.J.; Fernández Castillo, Noelia; Young, Andrew M.J. (2022-05-02)
      Article
      Open Access
      Genetic variants in YWHAZ contribute to psychiatric disorders such as autism spectrum disorder and schizophrenia, and have been related to an impaired neurodevelopment in humans and mice. Here, we have used zebrafish to ...

    • Development of microscopic techniques for the visualization of plant–root-knot nematode interaction 

      Vernet Pérez, Helena; Fullana Pons, Aïda Magdalena; Sorribas Royo, Francisco Javier; Gualda Manzano, Emilio José (2022-04-26)
      Article
      Open Access
      Plant-parasitic nematodes are a significant cause of yield losses and food security issues. Specifically, nematodes of the genus Meloidogyne can cause significant production losses in horticultural crops around the world. ...

    • Dynamics of macrophage polarization support Salmonella persistence in a whole living organism 

      Bernardello, Matteo; Gualda Manzano, Emilio José; Loza Álvarez, Pablo; Leiba, Jade; Sipka, Tamara; Began Pescia, Christina; Tairi, Sofiane; Bossi, Lionello; Gonzalez, Anne Alicia; Blanc Potard, Anne; Lutfalle, Georges; Nguyen Chi, Mai E (2024-01-15)
      Article
      Open Access
      The spatiotemporal dynamics of macrophage polarization during infection within host remain to be investigated. Here, we implement a model of persistent Salmonella. Typhimurium infection in zebrafish, which allows visualization ...

    • Femtosecond infrared intrastromal ablation and backscattering-mode adaptive-optics multiphoton microscopy in chicken corneas 

      Roso Franco, Luis; Artal, P; Gualda Manzano, Emilio José; Martínez García, M. Carmen; Roso Franco, Luis (2011-11-01)
      Article
      Open Access
      The performance of femtosecond (fs) laser intrastromal ablation was evaluated with backscattering-mode adaptive-optics multiphoton microscopy in ex vivo chicken corneas. The pulse energy of ...

    • Going "open" with Mesoscopy: a new dimension on multi-view imaging 

      Gualda Manzano, Emilio José; Martins, Gabriel G.; Moreno, Nuno; Tomancak, Pavel (2014-03)
      Article
      Open Access
      OpenSPIM and OpenSpinMicroscopy emerged as open access platforms for Light Sheet and Optical Projection Imaging, often called as optical mesoscopy techniques. Both projects can be easily reproduced using comprehensive ...

    • High-throughput live imaging using Light Sheet Microscopy 

      Gualda Manzano, Emilio José; Bernardello, Matteo; Marsal, Maria; Loza Álvarez, Pablo (2020)
      Conference report
      Open Access
      We have developed a compact, multi-modal lightsheet microscope platform where different kind of experiments may be addressed with a single equipment. We will evaluate the performance of the Flexi-SPIM platform and its ...