Now showing items 1-20 of 36

    • 3D volume rendering of invertebrates using light-sheet fluorescence microscopy 

      Gualda Manzano, Emilio José; Moreno, Nuno (2015-08)
      Article
      Open Access
      Light-Sheet Fluorescence Microscopy (LSFM) has recently emerged as the technique of choice for obtaining high quality 3D images of whole organisms with low photo-damage and fast acquisition rates. Unlike conventional ...
    • 3D-3-culture: A tool to unveil macrophage plasticity in the tumour microenvironment 

      Rebelo, Sofia P.; Martins, Tatiana R.; Estrada, Marta F.; Loza Álvarez, Pablo; Harrer, Nathalie; Alves, Paula M.; Cabeçadas, Jose; Gualda Manzano, Emilio José; Sommergruber, Wolfgang; Brito, Catarina (2018-05)
      Article
      Open Access
      The tumour microenvironment (TME) shapes disease progression and influences therapeutic response.Most aggressive solid tumours have high levels of myeloid cell infiltration, namely tumour associatedmacrophages (TAM). ...
    • A computational diffusion model to study antibody transport within reconstructed tumor microenvironments 

      Gualda Manzano, Emilio José; Marsal, Maria; Isidro, Inês; Almeida, Jaime (2020-12)
      Article
      Open Access
      Antibodies revolutionized cancer treatment over the past decades. Despite their successfully application, there are still challenges to overcome to improve efficacy, such as the heterogeneous distribution of antibodies ...
    • A novel culture method that sustains ERa signaling in human breast cancer tissue microstructures 

      Gualda Manzano, Emilio José; Furtado de Oliveira e Silva, Fernanda; Loza Álvarez, Pablo; Cartaxo, Ana Luisa; Estrada, Marta F.; Domenici, Giacomo; Sflomos, George; Brisken, Cathrin; Brito, Catarian (2020-12)
      Article
      Open Access
      Breast cancer (BC) is the most commonly diagnosedcancer among women worldwide [1]. It is a heteroge-neous disease with distinct biological features and clin-ical outcomes. Almost 75% of diagnosed BC expressestrogen ...
    • Adaptive optics multiphoton microscopy 

      Artal, P; Gualda Manzano, Emilio José; Bueno García, Juan Manuel (2010)
      Conference report
      Open Access
      We have developed an adaptive optics multiphoton microscope. The multiphoton imaging system combines an ultrafast high-power laser, a scanning unit, a motorized Z-scan device and a photon-counting detector. The adaptive ...
    • Adaptive optics multiphoton microscopy to study ex vivo ocular tissues 

      Bueno García, Juan Manuel; Gualda Manzano, Emilio José; Artal Soriano, Pablo (2010)
      Article
      Restricted access - publisher's policy
      We develop an adaptive optics (AO) multiphoton microscope by incorporating a deformable mirror and a Hartmann-Shack wavefront sensor. The AO module operating in closed-loop is used to correct for the aberrations of the ...
    • Analysis of aberrations effect in nonlinear processes for femtosecond laser pulses 

      Romero Altimis, C.; Gualda Manzano, Emilio José; Artal Soriano, Pablo; Bueno García, Juan Manuel; Borrego Varillas, Rocío; Alonso Fernández, Benjamín; Méndez Valverde, Cruz; Rodríguez Vázquez de Aldana, Javier; Roso Franco, Luis (Optical Society of American (OSA), 2009)
      Conference report
      Restricted access - publisher's policy
      A method for the analysis and control of femtosecond pulse wavefronts generated in non-linear crystals by three-wave mixing processes is presented. The possibility to improve the efficiency of these processes is discussed.
    • Analysis of corneal stroma organization with wavefront optimized nonlinear microscopy 

      Bueno García, Juan Manuel; Gualda Manzano, Emilio José; Artal Soriano, Pablo (2011-06)
      Article
      Open Access
      To investigate the organization of stromal collagen in healthy ex vivo corneas of different species from second harmonic generation (SHG) microscopy images
    • Analysis of the chicken retina with an adaptive optics multiphoton microscope 

      Gualda Manzano, Emilio José; Artal Soriano, Pablo; Bueno García, Juan Manuel; Giakoumaki, Anastasia; Schaeffel, Frank (2011-06-01)
      Article
      Restricted access - publisher's policy
      The structure and organization of the chicken retina has been investigated with an adaptive optics multiphoton imaging microscope in a backward configuration. Non-stained flat-mounted retinal tissues were imaged at ...
    • Compensation of third-order dispersion in a 100Gb/s single channel system with in-line fibre Bragg gratings 

      Gualda Manzano, Emilio José; Gómez Pavón, Luz del Carmen; Torres, Juan P. (2005-06-15)
      Article
      Open Access
      This paper presents a scheme to enhance the performance of an ultrahigh capacity(100 Gb/s) long haul transmission system that makes use of chirped fibre Bragggratings (FBG) for dispersion slope compensation. It is shown ...
    • Femtosecond infrared intrastromal ablation and backscattering-mode adaptive-optics multiphoton microscopy in chicken corneas 

      Roso Franco, Luis; Artal, P; Gualda Manzano, Emilio José; Martínez García, M. Carmen; Roso Franco, Luis (2011-11-01)
      Article
      Open Access
      The performance of femtosecond (fs) laser intrastromal ablation was evaluated with backscattering-mode adaptive-optics multiphoton microscopy in ex vivo chicken corneas. The pulse energy of ...
    • Going "open" with Mesoscopy: a new dimension on multi-view imaging 

      Gualda Manzano, Emilio José; Martins, Gabriel G.; Moreno, Nuno; Tomancak, Pavel (2014-03)
      Article
      Open Access
      OpenSPIM and OpenSpinMicroscopy emerged as open access platforms for Light Sheet and Optical Projection Imaging, often called as optical mesoscopy techniques. Both projects can be easily reproduced using comprehensive ...
    • High-throughput live imaging using Light Sheet Microscopy 

      Gualda Manzano, Emilio José; Bernardello, Matteo; Marsal, Maria; Loza Álvarez, Pablo (2020)
      Conference report
      Open Access
      We have developed a compact, multi-modal lightsheet microscope platform where different kind of experiments may be addressed with a single equipment. We will evaluate the performance of the Flexi-SPIM platform and its ...
    • Imaging of human differentiated 3D neural aggregates using light sheet fluorescence microscopy 

      Gualda Manzano, Emilio José; Brito, Catarina; Simão, Daniel; Pinto, Catarina; Alves, Paula M. (2014-08-06)
      Article
      Open Access
      The development of three dimensional (3D) cell cultures represents a big step for the better understanding of cell behavior and disease in a more natural like environment, providing not only single but multiple cell type ...
    • In vivo imaging of anatomical features of the nematode Caenorhabditis elegans using non-linear (TPEF-SHG-THG) microscopy 

      Gualda Manzano, Emilio José; Filippidis, George; Fotakis, Costas; Voglis, Giannis; Mari, Meropi; Tavernarakis, Nektarios (International Society for Photo-Optical Instrumentation Engineers (SPIE), 2007)
      Conference report
      Restricted access - publisher's policy
      In this study, we present the detailed imaging of the nematode Caenorhabditis elegans (C. elegans) at microscopic level by performing Two-Photon Excitation Fluorescence (TPEF), Second-Harmonic Generation (SHG) and Third ...
    • In vivo imaging of cell morphology and cellular processes in Caenorhabditis elegans, using non-linear phenomena 

      Filippidis, George; Fotakis, Costas; Gualda Manzano, Emilio José; Tavernarakis, Nektarios; Troulinaki, Kostoula; Mari, Meropi (2009-12)
      Article
      Open Access
      We present the detailed imaging of structures and processes of the nematodeCaenorhabditis elegans(C. elegans) using non-linear microscopy. Complementary information about the anatomy of thenematode was collected by ...
    • In vivo imaging of cellular structures and processes in Caenorhabditis elegans, using non-linear microscopy 

      Filippidis, George; Fotakis, Costas; Gualda Manzano, Emilio José; Mari, Meropi; Voglis, Giannis; Vlachos, Evangelos; Tavernarakis, Alexandros (Institute of Electrical and Electronics Engineers (IEEE), 2008)
      Conference report
      Restricted access - publisher's policy
      We present the detailed imaging of structures and processes of the nematode Caenorhabditis elegans (C. elegans) using nonlinear microscopy (TPEF-SHG-THG). Consummative and specific information about the anatomy of the ...
    • In vivo imaging of cellular structures in Caenorhabditis elegans by combined TPEF, SHG and THG microscopy 

      Gualda Manzano, Emilio José; Filippidis, George; Fotakis, Costas; Tavernarakis, Alexandros; Voglis, Giannis (2008-01)
      Article
      Open Access
      In this study, we use combined two-photon excitationfluorescence (TPEF), second-harmonic generation (SHG) andthird-harmonic generation (THG) measurements to imagecellular structures of the nematodeCaenorhabditis ...
    • In vivo imaging of neurodegeneration in Caenorhabditis elegans by third harmonic generation microscopy 

      Filippidis, George; Fotakis, Costas; Gualda Manzano, Emilio José; Voglis, Giannis; Tavernarakis, Nektarios; Melessanaki, Kristalia (2008-10-31)
      Article
      Open Access
      In this study, neurodegeneration phenomena wereinvestigated, by performing third harmonic generationimaging measurements on the nematodeCaenorhabditiselegans,in vivo. Thein vivo, precise identification of thecontour ...
    • Light-sheet microscopy imaging of a whole cleared rat brain with Thy1-GFP transgene 

      Gualda Manzano, Emilio José; Pawloswka, Monika; Marzena, Stefanivk; Legutko, Diana; Matryba, Pawel; Koze, Paulina; Konopka, Witold (Nature, 2016-09)
      Article
      Open Access
      Whole-brain imaging with light-sheet fluorescence microscopy and optically cleared tissue is a new, rapidly developing research field. Whereas successful attempts to clear and image mouse brain have been reported, a ...