3D volume rendering of invertebrates using light-sheet fluorescence microscopy

Abstract

Light-Sheet Fluorescence Microscopy (LSFM) has recently emerged as the technique of choice for obtaining high quality 3D images of whole organisms with low photo-damage and fast acquisition rates. Unlike conventional optical microscopy or scanning electron microscopy systems it offers the possibility of obtaining multi-views of the sample by rotating it. Here we show that the use of light sheet microscopy for the analysis of invertebrates provides a fair compromise compared to scanning electron microscopy (SEM) in terms of resolution but avoiding some its drawbacks such as sample preparation or limited three-dimensional perspectives