Now showing items 21-40 of 50

    • Imaging of human differentiated 3D neural aggregates using light sheet fluorescence microscopy 

      Gualda Manzano, Emilio José; Brito, Catarina; Simão, Daniel; Pinto, Catarina; Alves, Paula M. (2014-08-06)
      Article
      Open Access
      The development of three dimensional (3D) cell cultures represents a big step for the better understanding of cell behavior and disease in a more natural like environment, providing not only single but multiple cell type ...
    • In vivo imaging of anatomical features of the nematode Caenorhabditis elegans using non-linear (TPEF-SHG-THG) microscopy 

      Gualda Manzano, Emilio José; Filippidis, George; Fotakis, Costas; Voglis, Giannis; Mari, Meropi; Tavernarakis, Nektarios (International Society for Photo-Optical Instrumentation Engineers (SPIE), 2007)
      Conference report
      Restricted access - publisher's policy
      In this study, we present the detailed imaging of the nematode Caenorhabditis elegans (C. elegans) at microscopic level by performing Two-Photon Excitation Fluorescence (TPEF), Second-Harmonic Generation (SHG) and Third ...
    • In vivo imaging of cell morphology and cellular processes in Caenorhabditis elegans, using non-linear phenomena 

      Filippidis, George; Fotakis, Costas; Gualda Manzano, Emilio José; Tavernarakis, Nektarios; Troulinaki, Kostoula; Mari, Meropi (2009-12)
      Article
      Open Access
      We present the detailed imaging of structures and processes of the nematodeCaenorhabditis elegans(C. elegans) using non-linear microscopy. Complementary information about the anatomy of thenematode was collected by ...
    • In vivo imaging of cellular structures and processes in Caenorhabditis elegans, using non-linear microscopy 

      Filippidis, George; Fotakis, Costas; Gualda Manzano, Emilio José; Mari, Meropi; Voglis, Giannis; Vlachos, Evangelos; Tavernarakis, Alexandros (Institute of Electrical and Electronics Engineers (IEEE), 2008)
      Conference report
      Restricted access - publisher's policy
      We present the detailed imaging of structures and processes of the nematode Caenorhabditis elegans (C. elegans) using nonlinear microscopy (TPEF-SHG-THG). Consummative and specific information about the anatomy of the ...
    • In vivo imaging of cellular structures in Caenorhabditis elegans by combined TPEF, SHG and THG microscopy 

      Gualda Manzano, Emilio José; Filippidis, George; Fotakis, Costas; Tavernarakis, Alexandros; Voglis, Giannis (2008-01)
      Article
      Open Access
      In this study, we use combined two-photon excitationfluorescence (TPEF), second-harmonic generation (SHG) andthird-harmonic generation (THG) measurements to imagecellular structures of the nematodeCaenorhabditis ...
    • In vivo imaging of neurodegeneration in Caenorhabditis elegans by third harmonic generation microscopy 

      Filippidis, George; Fotakis, Costas; Gualda Manzano, Emilio José; Voglis, Giannis; Tavernarakis, Nektarios; Melessanaki, Kristalia (2008-10-31)
      Article
      Open Access
      In this study, neurodegeneration phenomena wereinvestigated, by performing third harmonic generationimaging measurements on the nematodeCaenorhabditiselegans,in vivo. Thein vivo, precise identification of thecontour ...
    • Light-sheet fluorescence microscopy for the in vivo study of microtubule dynamics in the zebrafish embryo 

      Bernardello, Matteo; Marsal, Maria; Gualda Manzano, Emilio José; Loza Álvarez, Pablo (2021-10-01)
      Article
      Open Access
      During its first hours of development, the zebrafish embryo presents a large microtubule array in the yolk region, essential for its development. Despite of its size and dynamic behavior, this network has been studied only ...
    • Light-sheet microscopy imaging of a whole cleared rat brain with Thy1-GFP transgene 

      Gualda Manzano, Emilio José; Pawloswka, Monika; Marzena, Stefanivk; Legutko, Diana; Matryba, Pawel; Koze, Paulina; Konopka, Witold (Nature, 2016-09)
      Article
      Open Access
      Whole-brain imaging with light-sheet fluorescence microscopy and optically cleared tissue is a new, rapidly developing research field. Whereas successful attempts to clear and image mouse brain have been reported, a ...
    • Light-sheet microscopy: a tutorial 

      Olarte, Omar Eduardo; Andilla, Jordi; Gualda Manzano, Emilio José; Loza Álvarez, Pablo (Optica Publishing Group, 2018-03-31)
      Article
      Open Access
      This paper is intended to give a comprehensive review of light-sheet (LS) microscopy from an optics perspective. As such, emphasis is placed on the advantages that LS micro- scope configurations present, given the degree ...
    • Modelling biochemical gradients in vitro to control cell compartmentalization in a microengineered 3D model of the intestinal epithelium 

      Altay, Gizem; Gualda Manzano, Emilio José; Fernández-Majada, Vanesa; Batlle, Eduard; Loza Álvarez, Pablo; Martínez Fraiz, Elena; Abad Lázaro, Aina; Folch Eguren, Jordi; Insa Segarra, Claudia; Tosi, Sebastien (2022-09-07)
      Article
      Open Access
      Gradients of signaling pathways within the intestinal stem cell (ISC) niche are instrumental for cellular compartmentalization and tissue function, yet how are they sensed by the epithelium is still not fully understood. ...
    • Modelling the tumour microenvironment in long-term microencapsulated 3D co-cultures recapitulates phenotypic features of disease progression 

      Gualda Manzano, Emilio José; Estrada, Marta F.; Alves, Paula M.; Brito, Catarina (2016-02)
      Article
      Open Access
      3D cell tumour models are generated mainly in non-scalable culture systems, using bioactive scaffolds. Many of these models fail to reflect the complex tumour microenvironment and do not allow long-term monitoring of ...
    • Modular multimodal platform for classical and high throughput light sheet microscopy 

      Bernardello, Matteo; Gualda Manzano, Emilio José; Loza Álvarez, Pablo (Nature, 2022-02-04)
      Article
      Open Access
      Light-sheet fluorescence microscopy (LSFM) has become an important tool for biological and biomedical research. Although several illumination and detection strategies have been developed, the sample mounting still represents ...
    • Multiphoton imaging microscopy at deeper layers with adaptive optics control of spherical aberration 

      Bueno García, Juan Manuel; Palacios Higueras, Raquel; Gualda Manzano, Emilio José; Artal Soriano, Pablo; Skorsetz, Martin (2013-07-17)
      Article
      Open Access
      Despite the inherent confocality and optical sectioning capabilities of multiphoton microscopy, threedimensional (3-D) imaging of thick samples is limited by the specimen-induced aberrations. The combination of immersion ...
    • Multiphoton microscopy of ex vivo corneas after collagen cross-linking 

      Marcos Celestino, Susana; Gualda Manzano, Emilio José; Artal Soriano, Pablo; Bueno García, Juan Manuel; Giakoumaki, Anastasia; Pérez Merino, Pablo (2011-07-18)
      Article
      Open Access
      To investigate changes in the morphology of the corneal stroma after collagen cross-linking (CXL) treatment in bovine and porcine eyes using a nonlinear microscope providing both two-photon excitation fluorescence (TPEF) ...
    • Multiple asters organize the yolk microtubule network during dclk2-GFP zebrafish epiboly 

      Marsal, Maria; Bernardello, Matteo; Gualda Manzano, Emilio José; Loza, Pablo (Nature, 2022-03-08)
      Article
      Open Access
      It is known that the organization of microtubule (MT) networks in cells is orchestrated by subcellular structures named MT organizing centers (MTOCs). In this work, we use Light Sheet Fluorescence and Confocal Microscopy ...
    • Nonlinear 3D microscopy of ex vivo corneas 

      Gualda Manzano, Emilio José; Artal Soriano, Pablo; Bueno García, Juan Manuel (International Society for Photo-Optical Instrumentation Engineers (SPIE), 2010)
      Conference report
      Open Access
      A multiphoton microscope has been developed to investigate the sources of nonlinear fluorescence (TPEF) and second harmonic generation (SHG) in non-stained samples of ex-vivo corneas. Stacks of images from different depths ...
    • Nonlinear imaging and THz diagnostic tools in the service of cultural heritage 

      Filippidis, George; Massaouti, M.; Selimis, A.; Tzortzakis, S.; Manceau, J. M.; Gualda Manzano, Emilio José (2012-02)
      Article
      Restricted access - publisher's policy
      We present the use of novel nonlinear imaging, terahertz time-domain spectroscopy and imaging as powerful diagnostic tools for studies of works of art. It is shown that nonlinear imaging offers precise in-depth information, while ...
    • Nonlinear imaging microscopy techniques as diagnostic tools for art conservation studies 

      Filippidis, George; Fotakis, Costas; Gualda Manzano, Emilio José; Melessanaki, Kristalia (2008-02-01)
      Article
      Open Access
      The present results of the implementation of three-photon excitation fluorescence (3PEF) and third harmonicgeneration imaging measurements for the precise and nondestructive detection of natural and synthetic var-nish ...
    • OpenSpinMicroscopy: an open-source integrated microscopy platform 

      Gualda Manzano, Emilio José; Feijó Muñoz, Jesús; Martins, Gabriel G.; Almada, Pedro (2013-07)
      Article
      Restricted access - publisher's policy
      Light-sheet microscopy has recently emerged as the technique of choice for fast, high-resolution three-dimensional (3D) imaging of whole organisms, one that also features low photodamage1,2. Unlike confocal or multiphoton ...
    • Response to hypoxic preconditioning of glial cells from the roof of the fourth ventricle 

      Gualda Manzano, Emilio José; Loza Álvarez, Pablo; Becerra González, Merymar; Varman Duraira, Ragu; Ostos Valverde, Aline; Portillo Martínez, Wendy; Gómez González, Gabriela Berenice; Buffo, Annalisa; Martínez Torres, Ataúlfo (2020-07)
      Article
      Open Access
      The cerebellum harbors a specialized area on the roof of the fourth ventricle that is composed of glial cells and neurons that interface with the cerebrospinal fluid. This region includes the so-called ventromedial cord ...