Analysis of intracellular protein dynamics in living zebrafish embryos using light-sheet fluorescence single-molecule microscopy
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Cita com:
hdl:2117/366416
Tipus de documentArticle
Data publicació2021-10-01
Condicions d'accésAccés obert
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continguts d'aquesta obra estan subjectes a la llicència de Creative Commons
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Reconeixement-NoComercial-SenseObraDerivada 3.0 Espanya
ProjecteLASERLAB-EUROPE - The Integrated Initiative of European Laser Research Infrastructures (EC-H2020-871124)
ImageInLife - Training European Experts in Multilevel Bioimaging, Analysis and Modelling of Vertebrate Development and Disease (EC-H2020-721537)
ImageInLife - Training European Experts in Multilevel Bioimaging, Analysis and Modelling of Vertebrate Development and Disease (EC-H2020-721537)
Abstract
Single-molecule microscopy techniques have emerged as useful tools to image
individual molecules and analyze their dynamics inside cells, but their application has mostly
been restricted to cell cultures. Here, a light-sheet fluorescence microscopy setup is presented for
imaging individual proteins inside living zebrafish embryos. The optical configuration makes this
design accessible to many laboratories and a dedicated sample-mounting system ensures sample
viability and mounting flexibility. Using this setup, we have analyzed the dynamics of individual
glucocorticoid receptors, which demonstrates that this approach creates multiple possibilities for
the analysis of intracellular protein dynamics in intact living organisms.
CitacióBernardello, M. [et al.]. Analysis of intracellular protein dynamics in living zebrafish embryos using light-sheet fluorescence single-molecule microscopy. "Biomedical optics express", 1 Octubre 2021, vol. 12, núm. 10.
ISSN2156-7085
Versió de l'editorhttps://opg.optica.org/boe/fulltext.cfm?uri=boe-12-10-6205&id=458665
Altres identificadorshttps://pubmed.ncbi.nlm.nih.gov/34745730/
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boe-12-10-6205.pdf | 4,453Mb | Visualitza/Obre |