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Development of microscopic techniques for the visualization of plant–root-knot nematode interaction

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Vernet Pérez, Helena
Fullana Pons, Aïda MagdalenaMés informacióMés informació
Sorribas Royo, Francisco JavierMés informacióMés informacióMés informació
Gualda Manzano, Emilio JoséMés informacióMés informacióMés informació
Document typeArticle
Defense date2022-04-26
Rights accessOpen Access
Attribution-NonCommercial-NoDerivs 3.0 Spain
Except where otherwise noted, content on this work is licensed under a Creative Commons license : Attribution-NonCommercial-NoDerivs 3.0 Spain
ProjectESTRATEGIAS DE GESTION DE GERMOPLASMA VEGETAL RESISTENTE A MELOIDOGYNE PARA EVITAR LA SELECCION DE VIRULENCIA (AEI-AGL2017-89785-R)
RYC-2015-17935 (MINECO-RYC-2015-17935)
Abstract
Plant-parasitic nematodes are a significant cause of yield losses and food security issues. Specifically, nematodes of the genus Meloidogyne can cause significant production losses in horticultural crops around the world. Understanding the mechanisms of the ever-changing physiology of plant roots by imaging the galls induced by nematodes could provide a great insight into their control. However, infected roots are unsuitable for light microscopy investigation due to the opacity of plant tissues. Thus, samples must be cleared to visualize the interior of whole plants in order to make them transparent using clearing agents. This work aims to identify which clearing protocol and microscopy system is the most appropriate to obtain 3D images of tomato cv. Durinta and eggplant cv. Cristal samples infected with Meloidogyne incognita to visualize and study the root–nematode interaction. To that extent, two clearing solutions (BABB and ECi), combined with three different dehydration solvents (ethanol, methanol and 1-propanol), are tested. In addition, the advantages and disadvantages of alternative imaging techniques to confocal microscopy are analyzed by employing an experimental custom-made setup that combines two microscopic techniques, light sheet fluorescence microscopy and optical projection tomography, on a single instrument.
CitationVernet, H. [et al.]. Development of microscopic techniques for the visualization of plant–root-knot nematode interaction. "Plants-Basel", 26 Abril 2022, vol. 11, núm. 9, p. 1-15. 
URIhttp://hdl.handle.net/2117/367483
DOI10.3390/plants11091165
ISSN2223-7747
Publisher versionhttps://www.mdpi.com/2223-7747/11/9/1165
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  • GINEMQUAL - Gestió Integrada de Nematodes Fitoparàsits i dels Efectes sobre el Rendiment i Qualitat de la Collita - Articles de revista [39]
  • Doctorat en Tecnologia Agroalimentària i Biotecnologia - Articles de revista [67]
  • Departament d'Enginyeria Agroalimentària i Biotecnologia - Articles de revista [1.023]
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