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dc.contributor.authorCabré, Gisela
dc.contributor.authorGarrido-Charle, Aida
dc.contributor.authorMoreno, Miquel
dc.contributor.authorBosch, Miquel
dc.contributor.authorPorta-de-la-Riva, Montserrat
dc.contributor.authorKrieg, Michael
dc.contributor.authorGascón-Moya, Marta
dc.contributor.authorCamarero, Núria
dc.contributor.authorGelabert, Ricard
dc.contributor.authorLluch, José M.
dc.contributor.authorBusqué, Félix
dc.contributor.authorHernando, Jordi
dc.contributor.authorGorostiza, Pau
dc.contributor.authorAlibés, Ramon
dc.date.accessioned2019-12-04T17:14:51Z
dc.date.available2019-12-04T17:14:51Z
dc.date.issued2019-02-22
dc.identifier.citationCabré, G. [et al.]. Rationally designed azobenzene photoswitches for efficient two-photon neuronal excitation. "Nature Communications", 22 Febrer 2019, vol. 10, núm. 907.
dc.identifier.urihttp://hdl.handle.net/2117/173553
dc.description.abstractManipulation of neuronal activity using two-photon excitation of azobenzene photoswitches with near-infrared light has been recently demonstrated, but their practical use in neuronal tissue to photostimulate individual neurons with three-dimensional precision has been hampered by firstly, the low efficacy and reliability of NIR-induced azobenzene photoisomerization compared to one-photon excitation, and secondly, the short cis state lifetime of the two-photon responsive azo switches. Here we report the rational design based on theoretical calculations and the synthesis of azobenzene photoswitches endowed with both high two-photon absorption cross section and slow thermal back-isomerization. These compounds provide optimized and sustained two-photon neuronal stimulation both in lightscattering brain tissue and in Caenorhabditis elegans nematodes, displaying photoresponse intensities that are comparable to those achieved under one-photon excitation. This finding opens the way to use both genetically targeted and pharmacologically selective azobenzene photoswitches to dissect intact neuronal circuits in three dimensions.
dc.format.extent12 p.
dc.language.isoeng
dc.publisherNature
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectÀrees temàtiques de la UPC::Física
dc.subject.lcshNeurons
dc.subject.otherneuronal excitation
dc.titleRationally designed azobenzene photoswitches for efficient two-photon neuronal excitation
dc.typeArticle
dc.subject.lemacNeurones
dc.identifier.doi10.1038/s41467-019-08796-9
dc.description.peerreviewedPeer Reviewed
dc.relation.publisherversionhttps://www.nature.com/articles/s41467-019-08796-9
dc.rights.accessOpen Access
dc.description.versionPostprint (published version)
dc.relation.projectidinfo:eu-repo/grantAgreement/MINECO//SEV-2015-0522/ES/AGR-INSTITUTO DE CIENCIAS FOTONICAS/
local.citation.publicationNameNature Communications
local.citation.volume10
local.citation.number907


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