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Rationally designed azobenzene photoswitches for efficient two-photon neuronal excitation
dc.contributor.author | Cabré, Gisela |
dc.contributor.author | Garrido-Charle, Aida |
dc.contributor.author | Moreno, Miquel |
dc.contributor.author | Bosch, Miquel |
dc.contributor.author | Porta-de-la-Riva, Montserrat |
dc.contributor.author | Krieg, Michael |
dc.contributor.author | Gascón-Moya, Marta |
dc.contributor.author | Camarero, Núria |
dc.contributor.author | Gelabert, Ricard |
dc.contributor.author | Lluch, José M. |
dc.contributor.author | Busqué, Félix |
dc.contributor.author | Hernando, Jordi |
dc.contributor.author | Gorostiza, Pau |
dc.contributor.author | Alibés, Ramon |
dc.date.accessioned | 2019-12-04T17:14:51Z |
dc.date.available | 2019-12-04T17:14:51Z |
dc.date.issued | 2019-02-22 |
dc.identifier.citation | Cabré, G. [et al.]. Rationally designed azobenzene photoswitches for efficient two-photon neuronal excitation. "Nature Communications", 22 Febrer 2019, vol. 10, núm. 907. |
dc.identifier.uri | http://hdl.handle.net/2117/173553 |
dc.description.abstract | Manipulation of neuronal activity using two-photon excitation of azobenzene photoswitches with near-infrared light has been recently demonstrated, but their practical use in neuronal tissue to photostimulate individual neurons with three-dimensional precision has been hampered by firstly, the low efficacy and reliability of NIR-induced azobenzene photoisomerization compared to one-photon excitation, and secondly, the short cis state lifetime of the two-photon responsive azo switches. Here we report the rational design based on theoretical calculations and the synthesis of azobenzene photoswitches endowed with both high two-photon absorption cross section and slow thermal back-isomerization. These compounds provide optimized and sustained two-photon neuronal stimulation both in lightscattering brain tissue and in Caenorhabditis elegans nematodes, displaying photoresponse intensities that are comparable to those achieved under one-photon excitation. This finding opens the way to use both genetically targeted and pharmacologically selective azobenzene photoswitches to dissect intact neuronal circuits in three dimensions. |
dc.format.extent | 12 p. |
dc.language.iso | eng |
dc.publisher | Nature |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Spain |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ |
dc.subject | Àrees temàtiques de la UPC::Física |
dc.subject.lcsh | Neurons |
dc.subject.other | neuronal excitation |
dc.title | Rationally designed azobenzene photoswitches for efficient two-photon neuronal excitation |
dc.type | Article |
dc.subject.lemac | Neurones |
dc.identifier.doi | 10.1038/s41467-019-08796-9 |
dc.description.peerreviewed | Peer Reviewed |
dc.relation.publisherversion | https://www.nature.com/articles/s41467-019-08796-9 |
dc.rights.access | Open Access |
dc.description.version | Postprint (published version) |
dc.relation.projectid | info:eu-repo/grantAgreement/MINECO//SEV-2015-0522/ES/AGR-INSTITUTO DE CIENCIAS FOTONICAS/ |
local.citation.publicationName | Nature Communications |
local.citation.volume | 10 |
local.citation.number | 907 |