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dc.contributor.authorPujol Vila, F
dc.contributor.authorVigués Frantzen, Núria
dc.contributor.authorGuerrero Navarro, A
dc.contributor.authorJimenez, S
dc.contributor.authorGomez, D
dc.contributor.authorFernandez, M
dc.contributor.authorBori Dols, Jaume
dc.contributor.authorValles Malet, Bettina
dc.contributor.authorRiva Juan, Mª del Carmen
dc.contributor.authorMuñoz Berbel, F. Xavier
dc.contributor.authorMas Gordi, Jordi
dc.contributor.otherUniversitat Politècnica de Catalunya. Institut d'Investigació Tèxtil i Cooperació Industrial de Terrassa
dc.identifier.citationPujol-Vila, F., Vigués, N., Guerrero-Navarro, A., Jimenez, S., Gomez, D., Fernandez, M., Bori, J., Valles, A., Riva, M., Muñoz-Berbel, X., Mas, J. Paper-based chromatic toxicity bioassay by analysis of bacterial ferricyanide reduction. "Analytica chimica acta", 3 Març 2016, vol. 910, p. 60-67.
dc.description.abstractWater quality assessment requires a continuous and strict analysis of samples to guarantee compliance with established standards. Nowadays, the increasing number of pollutants and their synergistic effects lead to the development general toxicity bioassays capable to analyse water pollution as a whole. Current general toxicity methods, e.g. Microtox®, rely on long operation protocols, the use of complex and expensive instrumentation and sample pre-treatment, which should be transported to the laboratory for analysis. These requirements delay sample analysis and hence, the response to avoid an environmental catastrophe. In an attempt to solve it, a fast (15 min) and low-cost toxicity bioassay based on the chromatic changes associated to bacterial ferricyanide reduction is here presented. E. coli cells (used as model bacteria) were stably trapped on low-cost paper matrices (cellulose-based paper discs, PDs) and remained viable for long times (1 month at -20 °C). Apart from bacterial carrier, paper matrices also acted as a fluidic element, allowing fluid management without the need of external pumps. Bioassay evaluation was performed using copper as model toxic agent. Chromatic changes associated to bacterial ferricyanide reduction were determined by three different transduction methods, i.e. (i) optical reflectometry (as reference method), (ii) image analysis and (iii) visual inspection. In all cases, bioassay results (in terms of half maximal effective concentrations, EC50) were in agreement with already reported data, confirming the good performance of the bioassay. The validation of the bioassay was performed by analysis of real samples from natural sources, which were analysed and compared with a reference method (i.e. Microtox). Obtained results showed agreement for about 70% of toxic samples and 80% of non-toxic samples, which may validate the use of this simple and quick protocol in the determination of general toxicity. The minimum instrumentation requirements and the simplicity of the bioassay open the possibility of in-situ water toxicity assessment with a fast and low-cost protocol
dc.format.extent8 p.
dc.subjectÀrees temàtiques de la UPC::Desenvolupament humà i sostenible::Degradació ambiental::Contaminació de l'aigua
dc.subject.lcshWater quality biological assessment
dc.subject.lcshToxicity testing
dc.subject.lcshBacterial pollution of water
dc.subject.lcshWater quality bioassay.
dc.subject.otherPaper-based toxicity bioassay
dc.subject.otherChromatic analysis
dc.subject.otherBacterial ferricyanide reduction
dc.subject.otherOptical reflectometry
dc.subject.otherNon-instrumental analysis
dc.subject.otherMicrobial respirometry
dc.titlePaper-based chromatic toxicity bioassay by analysis of bacterial ferricyanide reduction
dc.subject.lemacAigua -- Bioassaig de qualitat
dc.subject.lemacAigua -- Contaminació -- Anàlisi
dc.rights.accessOpen Access
dc.description.versionPostprint (author's final draft)
upcommons.citation.authorPujol-Vila, F., Vigués, N., Guerrero-Navarro, A., Jimenez, S., Gomez, D., Fernandez, M., Bori, J., Valles, A., Riva, M., Muñoz-Berbel, X., Mas, J.
upcommons.citation.publicationNameAnalytica chimica acta

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