Strain and Stress Analysis in Embryo Development
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hdl:2117/106736
Tipus de documentTreball Final de Grau
Data2016-10-11
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Abstract
In recent years, the technological evolution, that has taken place offering new
means for observation in biomedical research, along with the advance in the
knowledge of the genetic components that modulate embryonic process, open up
new paradigms of research that require appropriate processing and analysis
methods in order to allow a scientific interpretation. Therefore, currently one of
the most efficient technologies employed in this field is the confocal microscope,
which allows observation of microscopic samples in vivo genetically marked to give
fluorescence to cells of interest making an acquisition of several depth-cuts at each
interval of time. What is obtained then, an image in four dimensions, must be
analysed objectively through mathematical methods to provide a representative
model of the phenomenon which may involve behavioural patterns that develop in
tissues.
The images acquired can be used to trace embryo cells that are essential structures
for the origin and function of systems such as central nervous system (CNS) in the
case of glia, which in a given time they migrate to some specific positions.
Biologists have already been able to reveal interactions between cells and
molecular structures by morphology, gene expression and protein expression
marked with fluorescence, but not yet established a way to approximate
mechanical behaviour of the event.
The aim of this project is to design an automated cell-tracking code for glial cells,
which determine the constitution of the CNS during embryonic development of a
Drosophila melanogaster larvae, marked on acquisitions made with confocal
microscopy. Treating the larvae sample as a material which properties can be
modelled, material displacements of the points that move (strain) can be used for
studying stress occurring during the event. As a solution it’s proposed two
approaches to compute deformations automatically from 4D images.
MatèriesBiomedicina
TitulacióGRAU EN ENGINYERIA BIOMÈDICA (Pla 2009)
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MEMORIA.pdf | 3,357Mb | Accés restringit |