3D silicon doped hydroxyapatite scaffolds decorated with Elastin-like Recombinamers for bone regenerative medicine
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European Commisision's projectTHE GRAIL - Tissue in Host Engineering Guided Regeneration of Arterial Intimal Layer (EC-FP7-278557)
ELASTISLET - Tailored Elastin-like Recombinamers as Advanced Systems for Cell Therapies in Diabetes Mellitus: a Synthetic Biology Approach towards a Bioeffective and Immunoisolated Biosimilar Islet%2FCell Niche (EC-H2020-646075)
BIOGEL - Engineering responsive and biomimetic hydrogels for biomedical therapeutic and diagnostic applications (EC-H2020-642687)
The current study reports on the manufacturing by rapid prototyping technique of three-dimensional (3D) scaffolds based on silicon substituted hydroxyapatite with Elastin-like Recombinamers (ELRs) functionalized surfaces. Silicon doped hydroxyapatite (Si-HA), with Ca10(PO4)5.7(SiO4)0.3(OH)1.7h0.3 nominal formula, was surface functionalized with two different types of polymers designed by genetic engineering: ELR-RGD that contain cell attachment specific sequences and ELR-SNA15/RGD with both hydroxyapatite and cells domains that interact with the inorganic phase and with the cells, respectively. These hybrid materials were subjected to in vitro assays in order to clarify if the ELRs coating improved the well-known biocompatible and bone regeneration properties of calcium phosphates materials. The in vitro tests showed that there was a total and homogeneous colonization of the 3D scaffolds by Bone marrow Mesenchymal Stromal Cells (BMSCs). In addition, the BMSCs were viable and able to proliferate and differentiate into osteoblasts.
CitationVila, M., García, A., Girotti, A., Alonso, M., Rodriguez-Cabello , J.C., Gonzalez, A., Planell, J., Engel, E., Buján, J., García, N., Vallet-Regi, M. 3D silicon doped hydroxyapatite scaffolds decorated with Elastin-like Recombinamers for bone regenerative medicine. "Acta biomaterialia", 1 Novembre 2016, vol. 45, p. 349-356.