Escherichia coli and pseudomonas aeruginosa eradication by nano-penicillin G
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The transformation of penicillin G into nano/micro-sized spheres (nanopenicillin) using sonochemical technology was explored as a novel tool for the eradication of Gram-negative bacteria and their biofilms. Known by its effectiveness only against Gram-positive microorganisms, the penicillin G spherization boosted the inhibition of the Gram-negative Pseudomonas aeruginosa 10-fold (from 0.3 to 3.0 log-reduction) and additionally induced 1.2 log-reduction of Escherichia coli growth. The efficient penetration of the spheres within a Langmuir monolayer sustained the theory that nanopenicillin is able to cross the membrane and reach the periplasmic space in Gram-negative bacteria where they inhibit the ß-lactam targets: the transferases that build the bacteria cell wall. Moreover, it considerably suppressed the growth of both bacterial biofilms on a medically relevant polystyrene surface, leaving majority of the adhered cells dead compared to the treatment with the non-processed penicillin G. Importantly, nanopenicillin was found innocuous towards human fibroblasts at the antibacterial-effective concentration
CitationMacedo, M.M., Ivanova, K., Francesko, A., Rivera, D., Torrent, J.; Torrent-Burgués, J., Mendoza, E., Tzanov, T. Escherichia coli and pseudomonas aeruginosa eradication by nano-penicillin G. "Nanomedicine: nanotechnology, biology and medicine", 9 Juny 2016, vol. 12, núm. 7, p. 2061-2069.