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dc.contributor.authorViñuela Prieto, Jose Manuel
dc.contributor.authorSanchéz Quevedo, Maria del Carmen
dc.contributor.authorAlfonso-Rodriguez, Camilo
dc.contributor.authorXimenes Oliveira, Ana Celeste
dc.contributor.authorScionti, Giuseppe
dc.contributor.authorMartín-Piedra, Miguel Angel
dc.contributor.authorMoreu, Gerardo
dc.contributor.authorCampos, Antonio
dc.contributor.authorAlaminos, Miguel
dc.contributor.authorGarzón, Ingrid
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica
dc.identifier.citationViñuela-Prieto, J.M., Sanchéz-Quevedo, M.C., Alfonso, C.A., Oliveira, A.C., Scionti, G., Martín-Piedra, M.A., Moreu, G., Campos, A., Alaminos, M., Garzón, I. Sequential keratinocytic differentiation and maturation in a three-dimensional model of human artificial oral mucosa. "Journal of periodontal research", Octubre 2015.
dc.description.abstractDupuytren's disease is a fibro-proliferative disease characterized by a disorder of the extracellular matrix (ECM) and high myofibroblast proliferation. However, studies failed to determine if the whole palm fascia is affected by the disease. The objective of this study was to analyze several components of the extracellular matrix of three types of tissues—Dupuytren's diseased contracture cords (DDC), palmar fascia clinically unaffected by Dupuytren's disease contracture (NPF), and normal forehand fascia (NFF). Histological analysis, quantification of cells recultured from each type of tissue, mRNA microarrays and immunohistochemistry for smooth muscle actin (SMA), fibrillar ECM components and non-fibrillar ECM components were carried out. The results showed that DDC samples had abundant fibrosis with reticular fibers and few elastic fibers, high cell proliferation and myofibroblasts, laminin and glycoproteins, whereas NFF did not show any of these findings. Interestingly, NPF tissues had more cells showing myofibroblasts differentiation and more collagen and reticular fibers, laminin and glycoproteins than NFF, although at lower level than DDC, with similar elastic fibers than DDC. Immunohistochemical expression of decorin was high in DDC, whereas versican was highly expressed NFF, with no differences for aggrecan. Cluster analysis revealed that the global expression profile of NPF was very similar to DDC, and reculturing methods showed that cells corresponding to DDC tissues proliferated more actively than NPF, and NPF more actively than NFF. All these results suggest that NPF tissues may be affected, and that a modification of the therapeutic approach used for the treatment of Dupuytren's disease should be considered.
dc.subjectÀrees temàtiques de la UPC::Enginyeria dels materials
dc.subject.lcshTissue engineering
dc.titleSequential keratinocytic differentiation and maturation in a three-dimensional model of human artificial oral mucosa
dc.subject.lemacEnginyeria de teixits
dc.description.peerreviewedPeer Reviewed
dc.rights.accessOpen Access
dc.description.versionPostprint (published version)
upcommons.citation.authorViñuela-Prieto, J.M.; Sanchéz-Quevedo, M.C.; Alfonso, C.A.; Oliveira, A.C.; Scionti, G.; Martín-Piedra, M.A.; Moreu, G.; Campos, A.; Alaminos, M.; Garzón, I.
upcommons.citation.publicationNameJournal of periodontal research

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