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dc.contributor.authorHerranz-Diez, Carolina
dc.contributor.authorLi, Q
dc.contributor.authorLamprecht, C
dc.contributor.authorMas Moruno, Carlos
dc.contributor.authorNeubauer, Stefanie
dc.contributor.authorKessler, Horst
dc.contributor.authorManero Planella, José María
dc.contributor.authorGuillem Martí, Jordi
dc.contributor.authorSelhuber-Unkel, C
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica
dc.date.accessioned2016-03-08T11:03:12Z
dc.date.available2017-12-01T01:30:23Z
dc.date.issued2015
dc.identifier.citationHerranz-Diez, C., Li, Q., Lamprecht, C., Mas-Moruno, C., Neubauer, S., Kessler, H., Manero, J., Guillem-Marti, J., Selhuber-Unkel, C. Bioactive compounds immobilized on Ti and TiNbHf: AFM-based investigations of biofunctionalization efficiency and cell adhesion. "Colloids and Surfaces B. Biointerfaces", 2015, vol. 136, p. 704-711.
dc.identifier.issn0927-7765
dc.identifier.urihttp://hdl.handle.net/2117/83956
dc.description.abstractImplant materials require optimal biointegration, including strong and stable cell-material interactions from the early stages of implantation. Ti-based alloys with low elastic modulus are attracting a lot of interest for avoiding stress shielding, but their osseointegration potential is still very low. In this study, we report on how cell adhesion is influenced by linear RGD, cyclic RGD, and recombinant fibronectin fragment III8–10 coated on titanium versus a novel low-modulus TiNbHf alloy. The bioactive molecules were either physisorbed or covalently coupled to the substrates and their conformation on the surfaces was investigated with atomic force microscopy (AFM). The influence of the different bioactive coatings on the adhesion of rat mesenchymal stem cells was evaluated using cell culture assays and quantitatively analyzed at the single cell level by AFM-based single-cell force spectroscopy. Our results show that bioactive moieties, particularly fibronectin fragment III8–10, improve cell adhesion on titanium and TiNbHf and that the covalent tethering of such molecules provides the most promising strategy to biofunctionalize these materials. Therefore, the use of recombinant protein fragments is of high importance for improving the osseointegration potential of implant materials
dc.format.extent8 p.
dc.language.isoeng
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectÀrees temàtiques de la UPC::Enginyeria dels materials
dc.subject.lcshTitanium alloys
dc.subject.lcshBiomedical materials
dc.subject.lcshImplants, Artificial--Materials
dc.subject.otherTitanium alloy
dc.subject.otherRecombinant protein
dc.subject.otherRGD peptide
dc.subject.otherCell adhesion
dc.subject.otherAtomic forcemicroscopy
dc.subject.otherMesenchymal stem cell
dc.titleBioactive compounds immobilized on Ti and TiNbHf: AFM-based investigations of biofunctionalization efficiency and cell adhesion
dc.typeArticle
dc.subject.lemacTitani -- Aliatges
dc.subject.lemacImplants artificials -- Materials
dc.subject.lemacMaterials biomèdics
dc.contributor.groupUniversitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits
dc.identifier.doi10.1016/j.colsurfb.2015.10.008
dc.description.peerreviewedPeer Reviewed
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0927776515302393
dc.rights.accessOpen Access
drac.iddocument17545196
dc.description.versionPostprint (author's final draft)
dc.contributor.covenanteeTechnische Universität München
upcommons.citation.authorHerranz-Diez, C., Li, Q., Lamprecht, C., Mas-Moruno, C., Neubauer, S., Kessler, H., Manero, J., Guillem-Marti, J., Selhuber-Unkel, C.
upcommons.citation.publishedtrue
upcommons.citation.publicationNameColloids and Surfaces B. Biointerfaces
upcommons.citation.volume136
upcommons.citation.startingPage704
upcommons.citation.endingPage711


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