Show simple item record

dc.contributor.authorLlacher Alsina, Anna Pilar
dc.contributor.authorVallmitjana Lees, Alexander
dc.contributor.authorBenítez Iglesias, Raúl
dc.contributor.authorMontiel Dacosta, José Antonio
dc.contributor.authorCinca Cusculluela, Juan
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament d'Enginyeria de Sistemes, Automàtica i Informàtica Industrial
dc.date.accessioned2016-02-05T10:11:16Z
dc.date.available2016-02-05T10:11:16Z
dc.date.issued2016-01-01
dc.identifier.citationLlacher Alsina, Anna Pilar, Vallmitjana, A., Benitez, R., Montiel, J., Cinca, J. Prevention of adenosine A2A receptor activation diminishes beat-to-beat alternation in human atrial myocytes. "Basic research in cardiology", 01 Gener 2016, vol. 111, p. 1.
dc.identifier.issn0300-8428
dc.identifier.urihttp://hdl.handle.net/2117/82603
dc.description.abstract© 2015, Springer-Verlag Berlin Heidelberg. Atrial fibrillation (AF) has been associated with increased spontaneous calcium release from the sarcoplasmic reticulum and linked to increased adenosine A2A receptor (A2AR) expression and activation. Here we tested whether this may favor atrial arrhythmogenesis by promoting beat-to-beat alternation and irregularity. Patch-clamp and confocal calcium imaging was used to measure the beat-to-beat response of the calcium current and transient in human atrial myocytes. Responses were classified as uniform, alternating or irregular and stimulation of Gs-protein coupled receptors decreased the frequency where a uniform response could be maintained from 1.0 ± 0.1 to 0.6 ± 0.1 Hz; p < 0.01 for beta-adrenergic receptors and from 1.4 ± 0.1 to 0.5 ± 0.1 Hz; p < 0.05 for A2ARs. The latter was linked to increased spontaneous calcium release and after-depolarizations. Moreover, A2AR activation increased the fraction of non-uniformly responding cells in HL-1 myocyte cultures from 19 ± 3 to 51 ± 9 %; p < 0.02, and electrical mapping in perfused porcine atria revealed that adenosine induced electrical alternans at longer cycle lengths, doubled the fraction of electrodes showing alternation, and increased the amplitude of alternations. Importantly, protein kinase A inhibition increased the highest frequency where uniform responses could be maintained from 0.84 ± 0.12 to 1.86 ± 0.11 Hz; p < 0.001 and prevention of A2AR-activation with exogenous adenosine deaminase selectively increased the threshold from 0.8 ± 0.1 to 1.2 ± 0.1 Hz; p = 0.001 in myocytes from patients with AF. In conclusion, A2AR-activation promotes beat-to-beat irregularities in the calcium transient in human atrial myocytes, and prevention of A2AR activation may be a novel means to maintain uniform beat-to-beat responses at higher beating frequencies in patients with atrial fibrillation.
dc.format.extent1 p.
dc.language.isoeng
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectÀrees temàtiques de la UPC::Enginyeria biomèdica::Electrònica biomèdica
dc.subject.lcshMedical electronics
dc.subject.otherAdenosine receptor
dc.subject.otherAtrial myocyte
dc.subject.otherElectrophysiology
dc.subject.otherl-Type calcium current
dc.subject.otherSarcoplasmic reticulum
dc.titlePrevention of adenosine A2A receptor activation diminishes beat-to-beat alternation in human atrial myocytes
dc.typeArticle
dc.subject.lemacElectrònica mèdica
dc.contributor.groupUniversitat Politècnica de Catalunya. SISBIO - Senyals i Sistemes Biomèdics
dc.identifier.doi10.1007/s00395-015-0525-2
dc.rights.accessOpen Access
local.identifier.drac17420277
dc.description.versionPostprint (author's final draft)
local.citation.authorLlacher Alsina, Anna Pilar; Vallmitjana, A.; Benitez, R.; Montiel, J.; Cinca, J.
local.citation.publicationNameBasic research in cardiology
local.citation.volume111
local.citation.startingPage1
local.citation.endingPage1


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivs 3.0 Spain
Except where otherwise noted, content on this work is licensed under a Creative Commons license : Attribution-NonCommercial-NoDerivs 3.0 Spain