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dc.contributor.authorMartínez, Elena
dc.contributor.authorEngel López, Elisabeth
dc.contributor.authorLópez-Iglesias, Carmen
dc.contributor.authorMills, Christopher A
dc.contributor.authorPlanell Estany, Josep Anton
dc.contributor.authorSamitier Martí, Josep
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica
dc.date.accessioned2010-07-12T09:10:54Z
dc.date.available2010-07-12T09:10:54Z
dc.date.created2008
dc.date.issued2008
dc.identifier.citationMartínez, E. [et al.]. Focused ion beam/scanning electron microscopy characterization of cell behavior on polymer micro-/nanopatterned substrates: A study of cell¿substrate interactions. "Micron", 2008, vol. 39, núm. 2, p. 111-116.
dc.identifier.issn0968-4328
dc.identifier.urihttp://hdl.handle.net/2117/8120
dc.description.abstractTopographic micro and nanostructures can play an interesting role in cell behaviour when cells are cultured on these kinds of patterned substrates. It is especially relevant to investigate the influence of the nanometric dimensions topographic features on cell morphology, proliferation, migration and differentiation. To this end, some of the most recent fabrication technologies, developed for the microelectronics industry, can be used to produce well-defined micro and nanopatterns on biocompatible polymer substrates. In this work, osteoblast-like cells are grown on poly(methyl methacrylate) substrates patterned by nanoimprint lithography techniques. Examination of the cell–substrate interface can reveal important details about the cell morphology and the distribution of the focal contacts on the substrate surface. For this purpose, a combination of focused ion beam milling and scanning electron microscopy techniques has been used to image the cell–substrate interface. This technique, if applied to samples prepared by freeze-drying methods, allows high-resolution imaging of cross-sections through the cell and the substrate, where the interactions between the nanopatterned substrate, the cell and the extracellular matrix, which are normally hidden by the bulk of the cell, can be studied.
dc.format.extent6 p.
dc.language.isoeng
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectÀrees temàtiques de la UPC::Enginyeria dels materials
dc.subjectÀrees temàtiques de la UPC::Enginyeria dels materials::Metal·lúrgia
dc.subject.lcshOsteoblasts
dc.titleFocused ion beam/scanning electron microscopy characterization of cell behavior on polymer micro-/nanopatterned substrates: A study of cell¿substrate interactions
dc.typeArticle
dc.subject.lemacMaterials
dc.subject.lemacElectrònica
dc.contributor.groupUniversitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits
dc.identifier.doi10.1016/j.micron.2006.12.003
dc.rights.accessRestricted access - publisher's policy
local.identifier.drac2580975
dc.description.versionPostprint (published version)
local.citation.authorMartínez, E.; Engel, E.; López-Iglesias, C.; Mills, C.; Planell, J.; Samitier, J.
local.citation.publicationNameMicron
local.citation.volume39
local.citation.number2
local.citation.startingPage111
local.citation.endingPage116


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Attribution-NonCommercial-NoDerivs 3.0 Spain
Except where otherwise noted, content on this work is licensed under a Creative Commons license : Attribution-NonCommercial-NoDerivs 3.0 Spain