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Surface modifications of silicon nitride for cellular biosensor applications
dc.contributor.author | Gustavsson, Johan |
dc.contributor.author | Altankov, George Petrov |
dc.contributor.author | Errachid, Abdelhamid |
dc.contributor.author | Samitier Martí, Josep |
dc.contributor.author | Planell Estany, Josep Anton |
dc.contributor.author | Engel López, Elisabeth |
dc.contributor.other | Universitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica |
dc.date.accessioned | 2010-07-07T13:36:15Z |
dc.date.available | 2010-07-07T13:36:15Z |
dc.date.created | 2008-01 |
dc.date.issued | 2008-01 |
dc.identifier.citation | Gustavsson, J. [et al.]. Surface modifications of silicon nitride for cellular biosensor applications. "Journal of materials science. Materials in medicine", Gener 2008, vol. 19, núm. 4, p. 1839-1850. |
dc.identifier.issn | 0957-4530 |
dc.identifier.uri | http://hdl.handle.net/2117/8056 |
dc.description.abstract | Thin films of silicon nitride (Si3N4) can be used in several kinds of micro-sized biosensors as a material to monitor fine environmental changes related to the process of bone formation in vitro. We found however that Si3N4 does not provide optimal conditions for osseointegration as osteoblast-like MG-63 cells tend to detach from the surface when cultured over confluence. Therefore Si3N4 was modified with self-assembled monolayers bearing functional end groups of primary amine (NH2) and carboxyl (COOH) respectively. Both these modifications enhanced the interaction with confluent cell layers and thus improve osseointegration over Si3N4. Furthermore it was observed that the NH2 functionality increased the adsorption of fibronectin (FN), promoted cell proliferation, but delayed the differentiation. We also studied the fate of pre-adsorbed and secreted FN from cells to learn more about the impact of above functionalities for the development of provisional extracellular matrix on materials interface. Taken together our data supports that Si3N4 has low tissue integration but good cellular biocompatibility and thus is appropriate in cellular biosensor applications such as the ion-sensitive field effect transistor (ISFET). COOH and NH2 chemistries generally improve the interfacial tissue interaction with the sensor and they are therefore suitable substrates for monitoring cellular growth or matrix deposition using electrical impedance spectroscopy. |
dc.format.extent | 12 p. |
dc.language.iso | eng |
dc.subject | Àrees temàtiques de la UPC::Enginyeria biomèdica::Biomaterials |
dc.subject.lcsh | Biosensors--Materials |
dc.subject.lcsh | Thin films--Materials |
dc.subject.lcsh | Molecular biology |
dc.subject.lcsh | Monomolecular films |
dc.subject.lcsh | Silicon compounds |
dc.subject.lcsh | Tissue engineering |
dc.title | Surface modifications of silicon nitride for cellular biosensor applications |
dc.type | Article |
dc.subject.lemac | Biosensors |
dc.subject.lemac | Pel·lícules fines |
dc.subject.lemac | Biologia molecular |
dc.subject.lemac | Enginyeria de teixits |
dc.contributor.group | Universitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits |
dc.identifier.doi | 10.1007/s10856-008-3384-7 |
dc.rights.access | Restricted access - publisher's policy |
local.identifier.drac | 790780 |
dc.description.version | Postprint (published version) |
local.citation.author | Gustavsson, J.; Altankov, G.; Errachid, A.; Samitier, J.; Planell, J.; Engel, E. |
local.citation.publicationName | Journal of materials science. Materials in medicine |
local.citation.volume | 19 |
local.citation.number | 4 |
local.citation.startingPage | 1839 |
local.citation.endingPage | 1850 |
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