Hydraulic fracture during epithelial stretching
Rights accessOpen Access
European Commisision's projectGENESFORCEMOTION - Physical Forces Driving Collective Cell Migration: from Genes to Mechanism (EC-FP7-242993)
PREDMODSIM - Predictive models and simulations in nano- and biomolecular mechanics: a multiscale approach (EC-FP7-240487)
The origin of fracture in epithelial cell sheets subject to stretch is commonly attributed to excess tension in the cells' cytoskeleton, in the plasma membrane, or in cell-cell contacts. Here, we demonstrate that for a variety of synthetic and physiological hydrogel substrates the formation of epithelial cracks is caused by tissue stretching independently of epithelial tension. We show that the origin of the cracks is hydraulic; they result from a transient pressure build-up in the substrate during stretch and compression manoeuvres. After pressure equilibration, cracks heal readily through actomyosin-dependent mechanisms. The observed phenomenology is captured by the theory of poroelasticity, which predicts the size and healing dynamics of epithelial cracks as a function of the stiffness, geometry and composition of the hydrogel substrate. Our findings demonstrate that epithelial integrity is determined in a tension-independent manner by the coupling between tissue stretching and matrix hydraulics.
CitationCasares, L., Vincent, R., Zalvidea, D., Campillo, N., Navajas, D., Arroyo, M., Trepat, X. Hydraulic fracture during epithelial stretching. "Nature materials", 01 Març 2015, vol. 14, núm. 3, p. 343-351.