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dc.contributor.authorOlarte, Omar
dc.contributor.authorAndilla, Jordi
dc.contributor.authorArtigas García, David
dc.contributor.authorLoza-Alvarez, Pablo
dc.contributor.otherUniversitat Politècnica de Catalunya. Institut de Ciències Fotòniques
dc.date.accessioned2015-11-10T12:34:34Z
dc.date.issued2015-08-04
dc.identifier.issn2334-2536
dc.identifier.urihttp://hdl.handle.net/2117/78963
dc.description.abstractCurrent microscopy demands the visualization of large three-dimensional samples with increased sensitivity, higher resolution, and faster speed. Several imaging techniques based on widefield, point-scanning, and light-sheet strategies have been designed to tackle some of these demands. Although successful, all these require the illuminated volumes to be tightly coupled with the detection optics to accomplish efficient optical sectioning. Here, we break this paradigm and produce optical sections from out-of-focus planes. This is done by extending the depth of field of the detection optics in a light-sheet microscope using wavefront-coding techniques. This passive technique allows accommodation of the light sheet at any place within the extended axial range. We show that this enables quick scanning of the light sheet across a volumetric sample. As a consequence, imaging speeds faster than twice the volumetric video rate (>70  volumes/s) can be achieved without needing to move the sample. These capabilities are demonstrated for volumetric imaging of fast dynamics in vivo as well as for fast, three-dimensional particle tracking.
dc.format.extent4 p.
dc.language.isoeng
dc.publisherOSA
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectÀrees temàtiques de la UPC::Física
dc.subject.lcshImaging systems
dc.subject.otherimaging
dc.titleDecoupled illumination detection in light sheet microscopy for fast volumetric imaging
dc.typeArticle
dc.subject.lemacVisió
dc.description.peerreviewedPeer Reviewed
dc.relation.publisherversionhttps://www.osapublishing.org/optica/fulltext.cfm?uri=optica-2-8-702&id=323323
dc.rights.accessRestricted access - publisher's policy
dc.description.versionPostprint (published version)
dc.relation.projectidinfo:eu-repo/grantAgreement/EC/FP7/284464/EU/The Integrated Initiative of European Laser Research Infrastructures III/LASERLAB-EUROPE
dc.date.lift10000-01-01
local.citation.publicationNameOptica
local.citation.volume2
local.citation.number8
local.personalitzacitaciotrue


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