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dc.contributor.authorKalmar, Tibor
dc.contributor.authorLim, Chea
dc.contributor.authorHayward, Penelope
dc.contributor.authorMuñoz-Descalzo, Silvia
dc.contributor.authorNichols, Jennifer
dc.contributor.authorGarcía Ojalvo, Jordi
dc.contributor.authorMartinez-Arias, Alfonso
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament de Física i Enginyeria Nuclear
dc.date.accessioned2010-06-16T16:57:25Z
dc.date.available2010-06-16T16:57:25Z
dc.date.created2009-07-07
dc.date.issued2009-07-07
dc.identifier.citationKalmar, T. [et al.]. Regulated fluctuations in nanog expression mediate cell fate decisions in embryonic stem cells. "PLOS biology", 07 Juliol 2009, vol. 7, núm. 7, p. e1000149.
dc.identifier.issn1544-9173
dc.identifier.urihttp://hdl.handle.net/2117/7705
dc.description.abstractThere is evidence that pluripotency of mouse embryonic stem (ES) cells is associated with the activity of a network of transcription factors with Sox2, Oct4, and Nanog at the core. Using fluorescent reporters for the expression of Nanog, we observed that a population of ES cells is best described by a dynamic distribution of Nanog expression characterized by two peaks defined by high (HN) and low (LN) Nanog expression. Typically, the LN state is 5%–20% of the total population, depending on the culture conditions. Modelling of the activity of Nanog reveals that a simple network of Oct4/Sox2 and Nanog activity can account for the observed distribution and its properties as long as the transcriptional activity is tuned by transcriptional noise. The model also predicts that the LN state is unstable, something that is born out experimentally. While in this state, cells can differentiate. We suggest that transcriptional fluctuations in Nanog expression are an essential element of the pluripotent state and that the function of Sox2, Oct4, and Nanog is to act as a network that promotes and maintains transcriptional noise to interfere with the differentiation signals.
dc.language.isoeng
dc.rightsAttribution 3.0 Spain
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/
dc.subjectÀrees temàtiques de la UPC::Ciències de la salut::Medicina
dc.subject.lcshEmbryonic stem cells
dc.titleRegulated fluctuations in nanog expression mediate cell fate decisions in embryonic stem cells
dc.typeArticle
dc.subject.lemacCèl·lules mare -- Revistes
dc.contributor.groupUniversitat Politècnica de Catalunya. DONLL - Dinàmica no Lineal, Òptica no Lineal i Làsers
dc.identifier.doi10.1371/journal.pbio.1000149
dc.description.peerreviewedPeer Reviewed
dc.rights.accessOpen Access
local.identifier.drac2549266
dc.description.versionPostprint (published version)
local.citation.authorKalmar, T.; Lim, C.; Hayward, P.; Muñoz-Descalzo, S.; Nichols, J.; Garcia-Ojalvo, J.; Martinez-Arias, A.
local.citation.publicationNamePLOS biology
local.citation.volume7
local.citation.number7
local.citation.startingPagee1000149
local.citation.endingPagee1000149
dc.identifier.pmid19582141


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Attribution 3.0 Spain
Except where otherwise noted, content on this work is licensed under a Creative Commons license : Attribution 3.0 Spain