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dc.contributor.authorPérez Madrigal, Maria del Mar
dc.contributor.authorValle Mendoza, Luis Javier del
dc.contributor.authorArmelín Diggroc, Elaine Aparecida
dc.contributor.authorMichaux, Catherine
dc.contributor.authorRoussel, Guillaume
dc.contributor.authorPerpete, Eric
dc.contributor.authorAlemán Llansó, Carlos
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament d'Enginyeria Química
dc.date.accessioned2015-09-07T10:45:10Z
dc.date.available2016-01-01T01:30:45Z
dc.date.created2015-01-28
dc.date.issued2015-01-28
dc.identifier.citationPérez-Madrigal, M.M., del Valle, LJ., Armelin, E., Michaux, C., Roussel, G., Perpete, E., Aleman, C. Polypyrrole-Supported Membrane Proteins for Bio-Inspired Ion Channels. "ACS applied materials and interfaces", 28 Gener 2015, núm. 3, p. 1632-1643.
dc.identifier.issn1944-8244
dc.identifier.urihttp://hdl.handle.net/2117/76652
dc.description.abstractBiomedical platforms constructed by immobilizing membrane proteins in matrixes made of synthetic organic polymers is a challenge because the structure and function of these proteins are affected by environmental conditions. In this work, an operative composite that regulates the diffusion of alkali ions has been prepared by functionalizing a supporting matrix made of poly(N-methylpyrrole) (PNMPy) with a beta-barrel membrane protein (Omp2a) that forms channels and pores. The protein has been unequivocally identified in the composite, and its structure has been shown to remain unaltered. The PNMPy-Omp2a platform fulfills properties typically associated with functional bio-interfaces with biomedical applications (e.g., biocompatibility, biodegrabadility, and hydrophilicity). The functionality of the immobilized protein has been examined by studying the passive ion transport response in the presence of electrolytic solutions with Na+ and K+ concentrations close to those found in blood. Although the behavior of PNMPy and PNMPy-Omp2a is very similar for solutions with very low concentration, the resistance of the latter decreases drastically when the concentration of ions increases to similar to 100 mM. This reduction reflects an enhanced ion exchange between the biocomposite and the electrolytic medium, which is not observed in PNMPy, evidencing that PNMPy-Omp2a is particularly well suited to prepare bioinspired channels and smart biosensors.
dc.format.extent12 p.
dc.language.isoeng
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectÀrees temàtiques de la UPC::Enginyeria química
dc.subject.lcshPolymers in medicine
dc.subject.otherbioinspired channels
dc.subject.otherelectroactive polymer
dc.subject.otherelectrochemical impedance spectroscopy
dc.subject.otherion exchange
dc.subject.otherfunctional bio-interfaces
dc.subject.otherelectrochemical impedance spectroscopy
dc.subject.otherray photoelectron-spectroscopy
dc.subject.otherconducting polymers
dc.subject.otherbiomimetic membranes
dc.subject.othercopolymer
dc.subject.othercomposites
dc.subject.otherpoly(n-methylpyrrole)
dc.subject.otherderivatives
dc.subject.othermorphology
dc.subject.othernanotubes
dc.titlePolypyrrole-Supported Membrane Proteins for Bio-Inspired Ion Channels
dc.typeArticle
dc.subject.lemacPolímers
dc.subject.lemacBiopolímers
dc.contributor.groupUniversitat Politècnica de Catalunya. PSEP - Polimers Sintètics: Estructura i Propietats. Polimers Biodegradables.
dc.contributor.groupUniversitat Politècnica de Catalunya. IMEM - Innovació, Modelització i Enginyeria en (BIO) Materials
dc.identifier.doi10.1021/am507142f
dc.description.peerreviewedPeer Reviewed
dc.relation.publisherversionhttp://pubs.acs.org/
dc.rights.accessOpen Access
drac.iddocument15536753
dc.description.versionPostprint (author’s final draft)
upcommons.citation.authorPérez-Madrigal, M.M.; del Valle, LJ.; Armelin, E.; Michaux, C.; Roussel, G.; Perpete, E.; Aleman, C.
upcommons.citation.publishedtrue
upcommons.citation.publicationNameACS applied materials and interfaces
upcommons.citation.volume7
upcommons.citation.number3
upcommons.citation.startingPage1632
upcommons.citation.endingPage1643


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