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dc.contributor.authorGodoy Gallardo, Maria
dc.contributor.authorMas Moruno, Carlos
dc.contributor.authorYu, Kai
dc.contributor.authorManero Planella, José María
dc.contributor.authorGil Mur, Francisco Javier
dc.contributor.authorKizhakkedathu, Jayachandran N.
dc.contributor.authorRodríguez Rius, Daniel
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica
dc.date.accessioned2015-09-07T07:43:33Z
dc.date.available2015-09-07T07:43:33Z
dc.date.created2015-02-01
dc.date.issued2015-02-01
dc.identifier.citationGodoy, M., Mas-Moruno, C., Yu, K., Manero, J., Gil, F.J., Kizhakkedathu, J., Rodriguez, D. Antibacterial Properties of hLf1-11 Peptide onto Titanium Surfaces: A Comparison Study Between Silanization and Surface Initiated Polymerization. "Biomacromolecules", 01 Febrer 2015, núm. 2, p. 483-496.
dc.identifier.issn1525-7797
dc.identifier.urihttp://hdl.handle.net/2117/76643
dc.description.abstractDental implant failure can be associated with infections that develop into peri-implantitis. In order to reduce biofilm formation, several strategies focusing on the use of antimicrobial peptides (AMPs) have been studied. To covalently immobilize these molecules onto metallic substrates, several techniques have been developed, including silanization and polymer brush prepared by surface-initiated atom transfer radical polymerization (ATRP), with varied peptide binding yield and antibacterial performance. The aim of the present study was to compare the efficiency of these methods to immobilize the lactoferrin-derived hLf1-11 antibacterial peptide onto titanium, and evaluate their antibacterial activity in vitro. Smooth titanium samples were coated with hLf1-11 peptide under three different conditions: silanization with 3-aminopropyltriethoxysilane (APTES), and polymer brush based coatings with two different silanes. Peptide presence was determined by X-ray photoelectron spectroscopy, and the mechanical stability of the coatings was studied under ultrasonication. The LDH assays confirmed that HFFs viability and proliferation were no affected by the treatments. The in vitro antibacterial properties of the modified surfaces were tested with two oral strains (Streptococcus sanguinis and Lactobacillus salivarius) showing an outstanding reduction. A higher decrease in bacterial attachment was noticed when samples were modified by ATRP methods compared to silanization. This effect is likely due to the capacity to immobilize more peptide on the surfaces using polymer brushes and the nonfouling nature of polymer PDMA segment.
dc.format.extent14 p.
dc.language.isoeng
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectÀrees temàtiques de la UPC::Enginyeria dels materials
dc.subject.lcshDental Implants
dc.subject.lcshTitanium
dc.subject.othertransfer radical polymerization
dc.subject.otherantimicrobial peptides
dc.subject.otherescherichia-coli
dc.subject.otherstaphylococcus-aureus
dc.subject.otherlactoferricin-b
dc.subject.othercell-adhesive
dc.subject.otherin-vitro
dc.subject.otherattachment
dc.subject.otherroughness
dc.subject.otherbrushes
dc.titleAntibacterial Properties of hLf1-11 Peptide onto Titanium Surfaces: A Comparison Study Between Silanization and Surface Initiated Polymerization
dc.typeArticle
dc.subject.lemacTitani
dc.subject.lemacImplants artificials
dc.contributor.groupUniversitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits
dc.identifier.doi10.1021/bm501528x
dc.description.peerreviewedPeer Reviewed
dc.rights.accessOpen Access
local.identifier.drac15519560
dc.description.versionPostprint (author’s final draft)
local.citation.authorGodoy, M.; Mas-Moruno, C.; Yu, K.; Manero, J.; Gil, F.J.; Kizhakkedathu, J.; Rodriguez, D.
local.citation.publicationNameBiomacromolecules
local.citation.volume16
local.citation.number2
local.citation.startingPage483
local.citation.endingPage496


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Except where otherwise noted, content on this work is licensed under a Creative Commons license : Attribution-NonCommercial-NoDerivs 3.0 Spain