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Antibacterial Properties of hLf1-11 Peptide onto Titanium Surfaces: A Comparison Study Between Silanization and Surface Initiated Polymerization

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10.1021/bm501528x
 
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Godoy Gallardo, MariaMés informació
Mas Moruno, CarlosMés informacióMés informacióMés informació
Yu, Kai
Manero Planella, José MaríaMés informacióMés informacióMés informació
Gil Mur, Francisco JavierMés informacióMés informacióMés informació
Kizhakkedathu, Jayachandran N.
Rodríguez Rius, DanielMés informacióMés informacióMés informació
Document typeArticle
Defense date2015-02-01
Rights accessOpen Access
Attribution-NonCommercial-NoDerivs 3.0 Spain
This work is protected by the corresponding intellectual and industrial property rights. Except where otherwise noted, its contents are licensed under a Creative Commons license : Attribution-NonCommercial-NoDerivs 3.0 Spain
Abstract
Dental implant failure can be associated with infections that develop into peri-implantitis. In order to reduce biofilm formation, several strategies focusing on the use of antimicrobial peptides (AMPs) have been studied. To covalently immobilize these molecules onto metallic substrates, several techniques have been developed, including silanization and polymer brush prepared by surface-initiated atom transfer radical polymerization (ATRP), with varied peptide binding yield and antibacterial performance. The aim of the present study was to compare the efficiency of these methods to immobilize the lactoferrin-derived hLf1-11 antibacterial peptide onto titanium, and evaluate their antibacterial activity in vitro. Smooth titanium samples were coated with hLf1-11 peptide under three different conditions: silanization with 3-aminopropyltriethoxysilane (APTES), and polymer brush based coatings with two different silanes. Peptide presence was determined by X-ray photoelectron spectroscopy, and the mechanical stability of the coatings was studied under ultrasonication. The LDH assays confirmed that HFFs viability and proliferation were no affected by the treatments. The in vitro antibacterial properties of the modified surfaces were tested with two oral strains (Streptococcus sanguinis and Lactobacillus salivarius) showing an outstanding reduction. A higher decrease in bacterial attachment was noticed when samples were modified by ATRP methods compared to silanization. This effect is likely due to the capacity to immobilize more peptide on the surfaces using polymer brushes and the nonfouling nature of polymer PDMA segment.
CitationGodoy, M., Mas-Moruno, C., Yu, K., Manero, J., Gil, F.J., Kizhakkedathu, J., Rodriguez, D. Antibacterial Properties of hLf1-11 Peptide onto Titanium Surfaces: A Comparison Study Between Silanization and Surface Initiated Polymerization. "Biomacromolecules", 01 Febrer 2015, núm. 2, p. 483-496. 
URIhttp://hdl.handle.net/2117/76643
DOI10.1021/bm501528x
ISSN1525-7797
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  • Departament de Ciència dels Materials i Enginyeria Metal·lúrgica - Articles de revista [960]
  • BBT - Grup de recerca en Biomaterials, Biomecànica i Enginyeria de Teixits - Articles de revista [480]
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