Interdigitated μ-electrodes for development of an impedimetric immunosensor for atrazine detection.

Abstract

This contribution describes the development of an impedimetric immunosensor for atrazine detection. This immunosensor is based on the use of interdigitated metallic μ-electrodes (IDμEs) The method described in this work does not use any redox mediator and relies on the direct detection of immunochemical competitive reaction between the pesticide and a haptenized-protein immobilized on interdigitated μ-electrodes for the specific antibody. The immunoreagents used were specifically developed to detect atrazine. The immunochemical detection of this pesticide is achieved without using any label. The immunosensor shows a limit of detection of 8.34±1.37 μg L-1, witch is lower than the Maximun Residue Level (MRL) (50μg L-1)established by EU (European Union)for residues of atrazine as herbicide in the wine grapes and other foodstuff products.