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dc.contributor.authorGutierrez Capitan, Manuel
dc.contributor.authorSanchís Villariz, Ana
dc.contributor.authorCarvalho, Estela
dc.contributor.authorBaldi Coll, Antonio
dc.contributor.authorVilaplana Holgado, Lluïsa
dc.contributor.authorCardoso, Vanessa
dc.contributor.authorCalleja Navalpotro, Álvaro
dc.contributor.authorWei, Mingxing
dc.contributor.authorde la Rica, Roberto
dc.contributor.authorHoyo Pérez, Javier
dc.contributor.authorBassegoda Puigdomenech, Arnau
dc.contributor.authorTzanov, Tzanko
dc.contributor.authorMarco Colas, María Pilar
dc.contributor.authorLanceros Méndez, Senentxu
dc.contributor.authorFernandez Sanchez, Cesar
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament d'Enginyeria Química
dc.date.accessioned2023-10-04T12:15:07Z
dc.date.available2023-10-04T12:15:07Z
dc.date.issued2023-07-19
dc.identifier.citationGutierrez-Capitan, M. [et al.]. Engineering a point-of-care paper-microfluidic electrochemical device applied to the multiplexed quantitative detection of biomarkers in sputum. "ACS sensors", 19 Juliol 2023, vol. 8, núm. 8, p. 3032-3042.
dc.identifier.issn2379-3694
dc.identifier.urihttp://hdl.handle.net/2117/394601
dc.description.abstractHealth initiatives worldwide demand affordable point- of-care devices to aid in the reduction of morbidity and mortality rates of high-incidence infectious and noncommunicable diseases. However, the production of robust and reliable easy-to-use diagnostic platforms showing the ability to quantitatively measure several biomarkers in physiological fluids and that could in turn be decentralized to reach any relevant environment remains a challenge. Here, we show the particular combination of paper-microfluidic technology, electro- chemical transduction, and magnetic nanoparticle-based immunoassay approaches to produce a unique, compact, and easily deployable multiplex device to simultaneously measure interleukin-8, tumor necrosis factor-a, and myeloperoxidase biomarkers in sputum, developed with the aim of facilitating the timely detection of acute exacerbations of chronic obstructive pulmonary disease. The device incorporates an on-chip electrochemical cell array and a multichannel paper component, engineered to be easily aligned into a polymeric cartridge and exchanged if necessary. Calibration curves at clinically relevant biomarker concentration ranges are produced in buffer and artificial sputum. The analysis of sputum samples of healthy individuals and acutely exacerbated patients produces statistically significant biomarker concentration differences between the two studied groups. The device can be mass-produced at a low cost, being an easily adaptable platform for measuring other disease-related target biomarkers.
dc.description.sponsorshipThis research was funded by the Spanish Ministry of Economy and Competitiveness (MINECO) grants PCIN-2016-052 and PCIN-2016-134 and by Portuguese Fundação para a Ciência e a Technologia grant ENMed/0049/2016, through the ERA-NET EuroNanoMed II initiative (LungCheck project). This research was also funded by the European Commision-NextGenerationEU (Regulation EU 2020/2094), through CSIC’s Global Health Platform (PTI). This work used the Spanish ICTS Network MICRONANOFABS and was partly supported by the Spanish Ministry of Science and Innovation.
dc.format.extent11 p.
dc.language.isoeng
dc.publisherAmerican Chemical Society (ACS)
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectÀrees temàtiques de la UPC::Enginyeria química
dc.subject.lcshLungs -- Diseases, Obstructive
dc.subject.lcshBiosensors
dc.subject.lcshBioelectronics
dc.subject.otherPoint-of-care rapid tests
dc.subject.otherChronic obstructive pulmonary disease
dc.subject.otherElectrochemical biosensing
dc.subject.otherPaper microfluidics
dc.subject.otherMagnetic nanoparticle-based immunoassay
dc.subject.otherMultiplexed detection
dc.titleEngineering a point-of-care paper-microfluidic electrochemical device applied to the multiplexed quantitative detection of biomarkers in sputum
dc.typeArticle
dc.subject.lemacPulmons -- Malalties obstructives
dc.subject.lemacBiosensors
dc.subject.lemacBioelectrònica
dc.contributor.groupUniversitat Politècnica de Catalunya. GBMI - Grup de Biotecnologia Molecular i Industrial
dc.identifier.doi10.1021/acssensors.3c00523
dc.description.peerreviewedPeer Reviewed
dc.relation.publisherversionhttps://pubs.acs.org/doi/10.1021/acssensors.3c00523
dc.rights.accessOpen Access
local.identifier.drac36854274
dc.description.versionPostprint (published version)
local.citation.authorGutierrez-Capitan, M.; Sanchís, A.; Carvalho, E.; Baldi, A.; Vilaplana, L.; Cardoso, V.; Calleja, Á.; Wei, M.; de la Rica, R.; Hoyo, J.; Bassegoda, A.; Tzanov, T.; Marco, M.P.; Lanceros-Mendez, S.; Fernandez Sanchez, Cesar
local.citation.publicationNameACS sensors
local.citation.volume8
local.citation.number8
local.citation.startingPage3032
local.citation.endingPage3042


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