Chromosome-level assemblies from diverse clades reveal limited structural and gene content variation in the genome of Candida glabrata
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Cita com:
hdl:2117/377612
Tipus de documentArticle
Data publicació2022
EditorBioMed Central (BMC)
Condicions d'accésAccés obert
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continguts d'aquesta obra estan subjectes a la llicència de Creative Commons
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Reconeixement 4.0 Internacional
Abstract
Background
Candida glabrata is an opportunistic yeast pathogen thought to have a large genetic and phenotypic diversity and a highly plastic genome. However, the lack of chromosome-level genome assemblies representing this diversity limits our ability to accurately establish how chromosomal structure and gene content vary across strains.
Results
Here, we expanded publicly available assemblies by using long-read sequencing technologies in twelve diverse strains, obtaining a final set of twenty-one chromosome-level genomes spanning the known C. glabrata diversity. Using comparative approaches, we inferred variation in chromosome structure and determined the pan-genome, including an analysis of the adhesin gene repertoire. Our analysis uncovered four new adhesin orthogroups and inferred a rich ancestral adhesion repertoire, which was subsequently shaped through a still ongoing process of gene loss, gene duplication, and gene conversion.
Conclusions
C. glabrata has a largely stable pan-genome except for a highly variable subset of genes encoding cell wall-associated functions. Adhesin repertoire was established for each strain and showed variability among clades.
CitacióMarcet Houben, M. [et al.]. Chromosome-level assemblies from diverse clades reveal limited structural and gene content variation in the genome of Candida glabrata. "BMC Biology", 2022, vol. 20, núm. 1, 226.
ISSN1741-7007
Versió de l'editorhttps://bmcbiol.biomedcentral.com/articles/10.1186/s12915-022-01412-1
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s12915-022-01412-1.pdf | 1,840Mb | Visualitza/Obre |