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dc.contributor.authorHoyo, Javier
dc.contributor.authorBassegoda Puigdomenech, Arnau
dc.contributor.authorFerreres Cabanes, Guillem
dc.contributor.authorHinojosa Caballero, Dolores
dc.contributor.authorGutierrez Capitan, Manuel
dc.contributor.authorBaldi Coll, Antonio
dc.contributor.authorFernandez Sanchez, Cesar
dc.contributor.authorTzanov, Tzanko
dc.contributor.otherUniversitat Politècnica de Catalunya. Doctorat en Polímers i Biopolímers
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament d'Enginyeria Química
dc.date.accessioned2022-11-08T09:24:37Z
dc.date.available2022-11-08T09:24:37Z
dc.date.issued2022-08-15
dc.identifier.citationHoyo, J. [et al.]. Rapid colorimetric detection of wound infection with a fluidic paper device. "International journal of molecular sciences", 15 Agost 2022, vol. 23, núm. 16, article 9129, p. 1-10.
dc.identifier.issn1661-6596
dc.identifier.urihttp://hdl.handle.net/2117/375841
dc.description.abstractCurrent procedures for the assessment of chronic wound infection are time-consuming and require complex instruments and trained personnel. The incidence of chronic wounds worldwide, and the associated economic burden, urge for simple and cheap point-of-care testing (PoCT) devices for fast on-site diagnosis to enable appropriate early treatment. The enzyme myeloperoxidase (MPO), whose activity in infected wounds is about ten times higher than in non-infected wounds, appears to be a suitable biomarker for wound infection diagnosis. Herein, we develop a single-component foldable paper-based device for the detection of MPO in wound fluids. The analyte detection is achieved in two steps: (i) selective immunocapture of MPO, and (ii) reaction of a specific dye with the captured MPO, yielding a purple color with increasing intensity as a function of the MPO activity in infected wounds in the range of 20–85 U/mL. Ex vivo experiments with wound fluids validated the analytic efficiency of the paper-based device, and the results strongly correlate with a spectrophotometric assay.
dc.format.extent10 p.
dc.language.isoeng
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.lcshWounds and injuries -- Infections
dc.subject.lcshColorimetric analysis
dc.subject.otherColorimetric analysis
dc.subject.otherPoint-of-care device
dc.subject.otherMyeloperoxidase
dc.subject.otherChronic wounds
dc.subject.otherInfection biomarker
dc.titleRapid colorimetric detection of wound infection with a fluidic paper device
dc.typeArticle
dc.subject.lemacFerides i lesions -- Complicacions
dc.subject.lemacMicrobiologia mèdica
dc.contributor.groupUniversitat Politècnica de Catalunya. GBMI - Grup de Biotecnologia Molecular i Industrial
dc.identifier.doi10.3390/ijms23169129
dc.description.peerreviewedPeer Reviewed
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/23/16/9129
dc.rights.accessOpen Access
local.identifier.drac34181102
dc.description.versionPostprint (published version)
dc.relation.projectidinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104111RB-I00/ES/RECUBRIMIENTOS DE HIDROGEL QUE CONTIENEN NANOPARTICULAS ANTIMICROBIANAS PARA COMBATIR INFECCIONES ASOCIADAS AL USO DE CATETERES POR BACTERIAS RESISTENTES A LOS ANTIBIOTICOS/
dc.relation.projectidinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación (PEICTI) 2013-2016/PCIN-2017-134/ES/DEPOSICION DE CAPA ATOMICA PARA EL CRECIMIENTO DE ABAJO-ARRIBA DE PELICULAS DE FASES MAX Y MXENOS A MEDIDA/
local.citation.authorHoyo, J.; Bassegoda, A.; Ferreres, G.; Hinojosa, D.; Gutierrez-Capitan, M.; Baldi, A.; Fernandez Sanchez, Cesar; Tzanov, T.
local.citation.publicationNameInternational journal of molecular sciences
local.citation.volume23
local.citation.number16, article 9129
local.citation.startingPage1
local.citation.endingPage10


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