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dc.contributor.authorVernet Pérez, Helena
dc.contributor.authorFullana Pons, Aïda Magdalena
dc.contributor.authorSorribas Royo, Francisco Javier
dc.contributor.authorGualda Manzano, Emilio José
dc.contributor.otherUniversitat Politècnica de Catalunya. Doctorat en Tecnologia Agroalimentària i Biotecnologia
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament d'Enginyeria Agroalimentària i Biotecnologia
dc.date.accessioned2022-05-18T10:32:14Z
dc.date.available2022-05-18T10:32:14Z
dc.date.issued2022-04-26
dc.identifier.citationVernet, H. [et al.]. Development of microscopic techniques for the visualization of plant–root-knot nematode interaction. "Plants-Basel", 26 Abril 2022, vol. 11, núm. 9, p. 1-15.
dc.identifier.issn2223-7747
dc.identifier.urihttp://hdl.handle.net/2117/367483
dc.description.abstractPlant-parasitic nematodes are a significant cause of yield losses and food security issues. Specifically, nematodes of the genus Meloidogyne can cause significant production losses in horticultural crops around the world. Understanding the mechanisms of the ever-changing physiology of plant roots by imaging the galls induced by nematodes could provide a great insight into their control. However, infected roots are unsuitable for light microscopy investigation due to the opacity of plant tissues. Thus, samples must be cleared to visualize the interior of whole plants in order to make them transparent using clearing agents. This work aims to identify which clearing protocol and microscopy system is the most appropriate to obtain 3D images of tomato cv. Durinta and eggplant cv. Cristal samples infected with Meloidogyne incognita to visualize and study the root–nematode interaction. To that extent, two clearing solutions (BABB and ECi), combined with three different dehydration solvents (ethanol, methanol and 1-propanol), are tested. In addition, the advantages and disadvantages of alternative imaging techniques to confocal microscopy are analyzed by employing an experimental custom-made setup that combines two microscopic techniques, light sheet fluorescence microscopy and optical projection tomography, on a single instrument.
dc.format.extent15 p.
dc.language.isoeng
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectÀrees temàtiques de la UPC::Enginyeria agroalimentària::Agricultura::Fitopatologia
dc.subject.lcshPlant nematodes
dc.subject.otherPlant 3D imaging
dc.subject.otherRoot-knot nematode
dc.subject.otherTissue clearing
dc.subject.otherLight sheet fluorescence microscopy
dc.subject.otherOptical projection tomography
dc.titleDevelopment of microscopic techniques for the visualization of plant–root-knot nematode interaction
dc.typeArticle
dc.subject.lemacNematodes fitoparàsits
dc.contributor.groupUniversitat Politècnica de Catalunya. GINEMQUAL - Gestió Integrada de Nematodes Fitoparàsits i dels Efectes sobre el Rendiment i Qualitat de la Collita
dc.identifier.doi10.3390/plants11091165
dc.relation.publisherversionhttps://www.mdpi.com/2223-7747/11/9/1165
dc.rights.accessOpen Access
local.identifier.drac33102437
dc.description.versionPostprint (published version)
dc.relation.projectidinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-89785-R/ES/ESTRATEGIAS DE GESTION DE GERMOPLASMA VEGETAL RESISTENTE A MELOIDOGYNE PARA EVITAR LA SELECCION DE VIRULENCIA/
dc.relation.projectidinfo:eu-repo/grantAgreement/MINECO//RYC-2015-17935/ES/RYC-2015-17935/
local.citation.authorVernet, H.; Fullana, A.; Sorribas, F.; Gualda Manzano, Emilio Jose
local.citation.publicationNamePlants-Basel
local.citation.volume11
local.citation.number9
local.citation.startingPage1
local.citation.endingPage15


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