Estimation of cellular traction forces in morphogenesis of capillaries through advanced material modeling of fibrous biopolymers
Visualitza/Obre
Estadístiques de LA Referencia / Recolecta
Inclou dades d'ús des de 2022
Cita com:
hdl:2117/367246
Correu electrònic de l'autorgabrielle.ahhingicloud.com
Tipus de documentTreball Final de Grau
Data2022-02-07
Condicions d'accésAccés obert
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Reconeixement-NoComercial-SenseObraDerivada 3.0 Espanya
Abstract
Vaculogenesis is a process during which endothelial cells grow to form a network of interconnected vessels, to provide oxygen and nutrients to surrounding tissues. Endothelial cells microenvironment has an important impact on their growth. To understand such impact, one of the environment factors is studied in this report, i.e the traction forces that endothelial cells apply on the extracellular matrix. Those forces can modify the course of the network development. Based on a previous study, a combination of experimental and numerical approaches has been replicated for endothelial cells specifically in a three-dimension (3D) fashion. Fluorescence imaging was used to measure displacement fields with a fast iterative digital volume correlation (FIDVC). Then, forces applied on cells were determined with the technique of Traction Force Microscopy (TFM) using the Finite Element Method (FEM). The results showed that with a linear relationship between rigidity and density of the extracellular matrix, tractions, displacements, and density are independent. Furthermore, the independence between tractions, displacements and matrix density paves the way for more complex scenarios regarding the effect of matrix material remodeling.
TitulacióMOBILITAT INCOMING
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TFE_Ah-Hing_Gabrielle.pdf | 1,992Mb | Visualitza/Obre |