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dc.contributor.authorBernardello, Matteo
dc.contributor.authorGualda Manzano, Emilio José
dc.contributor.authorCastro Olvera, Gustavo
dc.contributor.authorLoza Álvarez, Pablo
dc.contributor.otherUniversitat Politècnica de Catalunya. Doctorat en Fotònica
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament d'Enginyeria Agroalimentària i Biotecnologia
dc.date.accessioned2022-04-27T11:07:36Z
dc.date.available2022-04-27T11:07:36Z
dc.date.issued2021-10-01
dc.identifier.citationBernardello, M. [et al.]. Analysis of intracellular protein dynamics in living zebrafish embryos using light-sheet fluorescence single-molecule microscopy. "Biomedical optics express", 1 Octubre 2021, vol. 12, núm. 10.
dc.identifier.issn2156-7085
dc.identifier.otherhttps://pubmed.ncbi.nlm.nih.gov/34745730/
dc.identifier.urihttp://hdl.handle.net/2117/366416
dc.description.abstractSingle-molecule microscopy techniques have emerged as useful tools to image individual molecules and analyze their dynamics inside cells, but their application has mostly been restricted to cell cultures. Here, a light-sheet fluorescence microscopy setup is presented for imaging individual proteins inside living zebrafish embryos. The optical configuration makes this design accessible to many laboratories and a dedicated sample-mounting system ensures sample viability and mounting flexibility. Using this setup, we have analyzed the dynamics of individual glucocorticoid receptors, which demonstrates that this approach creates multiple possibilities for the analysis of intracellular protein dynamics in intact living organisms.
dc.description.sponsorshipMinisterio de Economía y Competitividad (RYC-2015-17935, CEX2019-000910-S); Fundación Cellex (Mir-Puig); Laserlab-Europe (871124); Generalitat de Catalunya (CERCA); Fundación Cellex; Horizon 2020 Framework Programme (721537).
dc.language.isoeng
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectÀrees temàtiques de la UPC::Física
dc.subject.lcshFluorescence microscopy
dc.subject.lcshZebra danio
dc.titleAnalysis of intracellular protein dynamics in living zebrafish embryos using light-sheet fluorescence single-molecule microscopy
dc.typeArticle
dc.subject.lemacMicroscòpia de fluorescència
dc.identifier.doi10.1364/BOE.435103
dc.description.peerreviewedPeer Reviewed
dc.relation.publisherversionhttps://opg.optica.org/boe/fulltext.cfm?uri=boe-12-10-6205&id=458665
dc.rights.accessOpen Access
local.identifier.drac32106375
dc.description.versionPostprint (published version)
dc.relation.projectidinfo:eu-repo/grantAgreement/EC/H2020/871124/EU/The Integrated Initiative of European Laser Research Infrastructures/LASERLAB-EUROPE
dc.relation.projectidinfo:eu-repo/grantAgreement/EC/H2020/721537/EU/Training European Experts in Multilevel Bioimaging, Analysis and Modelling of Vertebrate Development and Disease/ImageInLife
local.citation.authorBernardello, M.; Gualda Manzano, Emilio Jose; Castro, G.; Loza, P.
local.citation.publicationNameBiomedical optics express
local.citation.volume12
local.citation.number10


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