Mostra el registre d'ítem simple
Analysis of intracellular protein dynamics in living zebrafish embryos using light-sheet fluorescence single-molecule microscopy
dc.contributor.author | Bernardello, Matteo |
dc.contributor.author | Gualda Manzano, Emilio José |
dc.contributor.author | Castro Olvera, Gustavo |
dc.contributor.author | Loza Álvarez, Pablo |
dc.contributor.other | Universitat Politècnica de Catalunya. Doctorat en Fotònica |
dc.contributor.other | Universitat Politècnica de Catalunya. Departament d'Enginyeria Agroalimentària i Biotecnologia |
dc.date.accessioned | 2022-04-27T11:07:36Z |
dc.date.available | 2022-04-27T11:07:36Z |
dc.date.issued | 2021-10-01 |
dc.identifier.citation | Bernardello, M. [et al.]. Analysis of intracellular protein dynamics in living zebrafish embryos using light-sheet fluorescence single-molecule microscopy. "Biomedical optics express", 1 Octubre 2021, vol. 12, núm. 10. |
dc.identifier.issn | 2156-7085 |
dc.identifier.other | https://pubmed.ncbi.nlm.nih.gov/34745730/ |
dc.identifier.uri | http://hdl.handle.net/2117/366416 |
dc.description.abstract | Single-molecule microscopy techniques have emerged as useful tools to image individual molecules and analyze their dynamics inside cells, but their application has mostly been restricted to cell cultures. Here, a light-sheet fluorescence microscopy setup is presented for imaging individual proteins inside living zebrafish embryos. The optical configuration makes this design accessible to many laboratories and a dedicated sample-mounting system ensures sample viability and mounting flexibility. Using this setup, we have analyzed the dynamics of individual glucocorticoid receptors, which demonstrates that this approach creates multiple possibilities for the analysis of intracellular protein dynamics in intact living organisms. |
dc.description.sponsorship | Ministerio de Economía y Competitividad (RYC-2015-17935, CEX2019-000910-S); Fundación Cellex (Mir-Puig); Laserlab-Europe (871124); Generalitat de Catalunya (CERCA); Fundación Cellex; Horizon 2020 Framework Programme (721537). |
dc.language.iso | eng |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Spain |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ |
dc.subject | Àrees temàtiques de la UPC::Física |
dc.subject.lcsh | Fluorescence microscopy |
dc.subject.lcsh | Zebra danio |
dc.title | Analysis of intracellular protein dynamics in living zebrafish embryos using light-sheet fluorescence single-molecule microscopy |
dc.type | Article |
dc.subject.lemac | Microscòpia de fluorescència |
dc.identifier.doi | 10.1364/BOE.435103 |
dc.description.peerreviewed | Peer Reviewed |
dc.relation.publisherversion | https://opg.optica.org/boe/fulltext.cfm?uri=boe-12-10-6205&id=458665 |
dc.rights.access | Open Access |
local.identifier.drac | 32106375 |
dc.description.version | Postprint (published version) |
dc.relation.projectid | info:eu-repo/grantAgreement/EC/H2020/871124/EU/The Integrated Initiative of European Laser Research Infrastructures/LASERLAB-EUROPE |
dc.relation.projectid | info:eu-repo/grantAgreement/EC/H2020/721537/EU/Training European Experts in Multilevel Bioimaging, Analysis and Modelling of Vertebrate Development and Disease/ImageInLife |
local.citation.author | Bernardello, M.; Gualda Manzano, Emilio Jose; Castro, G.; Loza, P. |
local.citation.publicationName | Biomedical optics express |
local.citation.volume | 12 |
local.citation.number | 10 |
Fitxers d'aquest items
Aquest ítem apareix a les col·leccions següents
-
Articles de revista [71]
-
Articles de revista [1.045]