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Hybrid conducting alginate-based hydrogel for hydrogen peroxide detection from enzymatic oxidation of lactate
dc.contributor.author | Babeli Aguilera, Ismael |
dc.contributor.author | Puiggalí Jou, Anna |
dc.contributor.author | Roa Rovira, Joan Josep |
dc.contributor.author | Ginebra Molins, Maria Pau |
dc.contributor.author | García Torres, José Manuel |
dc.contributor.author | Alemán Llansó, Carlos |
dc.contributor.other | Universitat Politècnica de Catalunya. Departament de Ciència i Enginyeria de Materials |
dc.contributor.other | Universitat Politècnica de Catalunya. Departament d'Enginyeria Química |
dc.date.accessioned | 2022-02-17T14:24:03Z |
dc.date.available | 2022-12-15T01:26:37Z |
dc.date.issued | 2021-12-15 |
dc.identifier.citation | Babeli, I. [et al.]. Hybrid conducting alginate-based hydrogel for hydrogen peroxide detection from enzymatic oxidation of lactate. "International journal of biological macromolecules", 15 Desembre 2021, vol. 193, p. 1237-1248. |
dc.identifier.issn | 1879-0003 |
dc.identifier.uri | http://hdl.handle.net/2117/362587 |
dc.description.abstract | A conducting nanocomposite hydrogel is developed for the detection of L-lactate. The hydrogel is based on a mixture of alginate (Alg) and poly(3,4-ethylenedioxythiophene) (PEDOT), which is loaded with gold nanoparticles (GNP). In this novel hydrogel, Alg provides 3D structural support and flexibility, PEDOT confers conductivity and sensing capacity, and GNP provides signal amplification with respect to simple voltammetric and chronoamperometric response. The synergistic combination of the properties provided by each component results in a new flexible nanocomposite with outstanding capacity to detect hydrogen peroxide, which has been used to detect the oxidation of L-lactate. The hydrogel detects hydrogen peroxide with linear response and limits of detection of 0.91 µM and 0.02 µM by cyclic voltammetry and chronoamperometry, respectively. The hydrogel is functionalized with lactate oxidase, which catalyzes the oxidation of L-lactate to pyruvate, forming hydrogen peroxide. For L-lactate detection, the functionalized biosensor works in two linear regimes, one for concentrations lower than 5 mM with a limit of detection of 0.4 mM, and the other for concentrations up to 100 mM with a limit of detection of 3.5 mM. Because of its linear range interval, the developed biosensor could be suitable for a wide number of biological fluids. |
dc.format.extent | 12 p. |
dc.language.iso | eng |
dc.publisher | Elsevier |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Spain |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ |
dc.subject | Àrees temàtiques de la UPC::Enginyeria química |
dc.subject | Àrees temàtiques de la UPC::Enginyeria dels materials |
dc.subject.lcsh | Biosensors |
dc.subject.other | Biosensor |
dc.subject.other | Enzymatic oxidation |
dc.subject.other | Lactate oxidase |
dc.title | Hybrid conducting alginate-based hydrogel for hydrogen peroxide detection from enzymatic oxidation of lactate |
dc.type | Article |
dc.subject.lemac | Biosensors |
dc.contributor.group | Universitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits |
dc.contributor.group | Universitat Politècnica de Catalunya. IMEM-BRT- Innovation in Materials and Molecular Engineering - Biomaterials for Regenerative Therapies |
dc.identifier.doi | 10.1016/j.ijbiomac.2021.10.169 |
dc.description.peerreviewed | Peer Reviewed |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/abs/pii/S0141813021023266 |
dc.rights.access | Open Access |
local.identifier.drac | 32510052 |
dc.description.version | Postprint (author's final draft) |
dc.contributor.covenantee | Institut de Bioenginyeria de Catalunya |
local.citation.author | Babeli, I.; Puiggalí, A.; Roa, J.; Ginebra, M.P.; Garcia-Torres, J.; Aleman, C. |
local.citation.publicationName | International journal of biological macromolecules |
local.citation.volume | 193 |
local.citation.startingPage | 1237 |
local.citation.endingPage | 1248 |
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