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dc.contributor.authorBabeli Aguilera, Ismael
dc.contributor.authorPuiggalí Jou, Anna
dc.contributor.authorRoa Rovira, Joan Josep
dc.contributor.authorGinebra Molins, Maria Pau
dc.contributor.authorGarcía Torres, José Manuel
dc.contributor.authorAlemán Llansó, Carlos
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament de Ciència i Enginyeria de Materials
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament d'Enginyeria Química
dc.date.accessioned2022-02-17T14:24:03Z
dc.date.available2022-12-15T01:26:37Z
dc.date.issued2021-12-15
dc.identifier.citationBabeli, I. [et al.]. Hybrid conducting alginate-based hydrogel for hydrogen peroxide detection from enzymatic oxidation of lactate. "International journal of biological macromolecules", 15 Desembre 2021, vol. 193, p. 1237-1248.
dc.identifier.issn1879-0003
dc.identifier.urihttp://hdl.handle.net/2117/362587
dc.description.abstractA conducting nanocomposite hydrogel is developed for the detection of L-lactate. The hydrogel is based on a mixture of alginate (Alg) and poly(3,4-ethylenedioxythiophene) (PEDOT), which is loaded with gold nanoparticles (GNP). In this novel hydrogel, Alg provides 3D structural support and flexibility, PEDOT confers conductivity and sensing capacity, and GNP provides signal amplification with respect to simple voltammetric and chronoamperometric response. The synergistic combination of the properties provided by each component results in a new flexible nanocomposite with outstanding capacity to detect hydrogen peroxide, which has been used to detect the oxidation of L-lactate. The hydrogel detects hydrogen peroxide with linear response and limits of detection of 0.91 µM and 0.02 µM by cyclic voltammetry and chronoamperometry, respectively. The hydrogel is functionalized with lactate oxidase, which catalyzes the oxidation of L-lactate to pyruvate, forming hydrogen peroxide. For L-lactate detection, the functionalized biosensor works in two linear regimes, one for concentrations lower than 5 mM with a limit of detection of 0.4 mM, and the other for concentrations up to 100 mM with a limit of detection of 3.5 mM. Because of its linear range interval, the developed biosensor could be suitable for a wide number of biological fluids.
dc.format.extent12 p.
dc.language.isoeng
dc.publisherElsevier
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectÀrees temàtiques de la UPC::Enginyeria química
dc.subjectÀrees temàtiques de la UPC::Enginyeria dels materials
dc.subject.lcshBiosensors
dc.subject.otherBiosensor
dc.subject.otherEnzymatic oxidation
dc.subject.otherLactate oxidase
dc.titleHybrid conducting alginate-based hydrogel for hydrogen peroxide detection from enzymatic oxidation of lactate
dc.typeArticle
dc.subject.lemacBiosensors
dc.contributor.groupUniversitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits
dc.contributor.groupUniversitat Politècnica de Catalunya. IMEM-BRT- Innovation in Materials and Molecular Engineering - Biomaterials for Regenerative Therapies
dc.identifier.doi10.1016/j.ijbiomac.2021.10.169
dc.description.peerreviewedPeer Reviewed
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/abs/pii/S0141813021023266
dc.rights.accessOpen Access
local.identifier.drac32510052
dc.description.versionPostprint (author's final draft)
dc.contributor.covenanteeInstitut de Bioenginyeria de Catalunya
local.citation.authorBabeli, I.; Puiggalí, A.; Roa, J.; Ginebra, M.P.; Garcia-Torres, J.; Aleman, C.
local.citation.publicationNameInternational journal of biological macromolecules
local.citation.volume193
local.citation.startingPage1237
local.citation.endingPage1248


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