Easily applicable modifications to electroporation conditions improve the transformation efficiency rates for rough morphotypes of fast-growing mycobacteria
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Cita com:
hdl:2117/359689
Tipus de documentArticle
Data publicació2021-07
EditorElsevier
Condicions d'accésAccés obert
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Reconeixement-NoComercial-SenseObraDerivada 3.0 Espanya
Abstract
Electroporation is the most widely used and efficient method to transform mycobacteria. Through this technique, fast- and slow-growing mycobacteria with smooth and rough morphotypes have been successfully transformed. However, transformation efficiencies differ widely between species and strains. In this study, the smooth and rough morphotypes of Mycobacteroides abscessus and Mycolicibacterium brumae were used to improve current electroporation procedures for fast-growing rough mycobacteria
CitacióCampo, V. [et al.]. Easily applicable modifications to electroporation conditions improve the transformation efficiency rates for rough morphotypes of fast-growing mycobacteria. "New biotechnology", Juliol 2021, vol. 63, p. 10-18.
ISSN1871-6784
Versió de l'editorhttps://www.sciencedirect.com/science/article/pii/S1871678421000273
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Postprint_New biotechnology.pdf | Main article + figures | 1,438Mb | Visualitza/Obre |