Pseudomonas aeruginosa host-pathogen interactions in human corneal infection models

Abstract

In the whole tissue model (WTM), donor buttons were infected with PAO1 by scoring and intrastromal in-jection. Tissue was examined after 3, 9 and 24 h post challenge by transmission electron microscopy (TEM) andscanning electron microscopy (SEM).In the cell culture model (CCM), cornealfibroblasts (CF) were infected in vitro with PAO1. Bacterial ad-herence and internalization were assayed at 3, 6 and 9 h by SEM and TEM. Adherent and internalized bacteriawere measured by the gentamicin protection invasion assay