Development and angiogenic potential of cell-derived microtissues using microcarrier-template
Visualitza/Obre
10.3390/biomedicines9030232
Inclou dades d'ús des de 2022
Cita com:
hdl:2117/351411
Tipus de documentArticle
Data publicació2021-02-25
Condicions d'accésAccés obert
Llevat que s'hi indiqui el contrari, els
continguts d'aquesta obra estan subjectes a la llicència de Creative Commons
:
Reconeixement-NoComercial-SenseObraDerivada 4.0 Internacional
Abstract
Tissue engineering and regenerative medicine approaches use biomaterials in combination with cells to regenerate lost functions of tissues and organs to prevent organ transplantation. However, most of the current strategies fail in mimicking the tissue’s extracellular matrix properties. In order to mimic native tissue conditions, we developed cell-derived matrix (CDM) microtissues (MT). Our methodology uses poly-lactic acid (PLA) and Cultispher® S microcarriers’ (MCs’) as scaffold templates, which are seeded with rat bone marrow mesenchymal stem cells (rBM-MSCs). The scaffold template allows cells to generate an extracellular matrix, which is then extracted for downstream use. The newly formed CDM provides cells with a complex physical (MT architecture) and biochemical (deposited ECM proteins) environment, also showing spontaneous angiogenic potential. Our results suggest that MTs generated from the combination of these two MCs (mixed MTs) are excellent candidates for tissue vascularization. Overall, this study provides a methodology for in-house fabrication of microtissues with angiogenic potential for downstream use in various tissue regenerative strategies.
CitacióRubi-Sans, G. [et al.]. Development and angiogenic potential of cell-derived microtissues using microcarrier-template. "Biomedicines", 25 Febrer 2021, vol. 9, núm. 3, p. 232:1-232:18.
ISSN2227-9059
Versió de l'editorhttps://www.mdpi.com/2227-9059/9/3/232
Fitxers | Descripció | Mida | Format | Visualitza |
---|---|---|---|---|
biomedicines-09-00232-v3.pdf | 6,157Mb | Visualitza/Obre |