Structural characterization of NORAD reveals a stabilizing role of spacers and two new repeat units
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Cita com:
hdl:2117/350514
Tipus de documentArticle
Data publicació2021
EditorElsevier
Condicions d'accésAccés obert
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continguts d'aquesta obra estan subjectes a la llicència de Creative Commons
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Reconeixement-NoComercial-SenseObraDerivada 4.0 Internacional
ProjecteMULTI_FOLDS - Not one but many: adopting structural flexibility in the analysis of the evolution of lncRNAs. (EC-H2020-793699)
RETVOLUTION - Reticulate evolution: patterns and impacts of non-vertical inheritance in eukaryotic genomes. (EC-H2020-724173)
RETVOLUTION - Reticulate evolution: patterns and impacts of non-vertical inheritance in eukaryotic genomes. (EC-H2020-724173)
Abstract
Long non-coding RNAs (lncRNAs) can perform a variety of key cellular functions by interacting with proteins and other RNAs. Recent studies have shown that the functions of lncRNAS are largely mediated by their structures. However, our structural knowledge for most lncRNAS is limited to sequence-based computational predictions. Non-coding RNA activated by DNA damage (NORAD) is an atypical lncRNA due to its abundant expression and high sequence conservation. NORAD regulates genomic stability by interacting with proteins and microRNAs. Previous sequence-based characterization has identified a modular organization of NORAD composed of several NORAD repeat units (NRUs). These units comprise the protein-binding elements and are separated by regular spacers. Here, we experimentally determine for the first time the secondary structure of NORAD using the nextPARS approach. Our results suggest that the spacer regions provide structural stability to NRUs. Furthermore, we uncover two previously unreported NRUs, and determine the core structural motifs conserved across NRUs. Overall, these findings will help to elucidate the function and evolution of NORAD.
CitacióChorostecki, U.; Saus, E.; Gabaldón, T. Structural characterization of NORAD reveals a stabilizing role of spacers and two new repeat units. "Computational and Structural Biotechnology Journal", 2021, vol. 19, p. 3245-3254.
ISSN2001-0370
Versió de l'editorhttps://www.sciencedirect.com/science/article/pii/S2001037021002269
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