Computed tomography and histological evaluation of xenogenic and biomimetic bone grafts in three-wall alveolar defects in minipigs
Visualitza/Obre
Cita com:
hdl:2117/348615
Realitzat a/ambMimetis Biomaterials; Universitat Internacional de Catalunya; Universitat de Barcelona. Departament de Patologia i Terapèutica Experimental
Tipus de documentArticle
Data publicació2021-05-01
Condicions d'accésAccés obert
Llevat que s'hi indiqui el contrari, els
continguts d'aquesta obra estan subjectes a la llicència de Creative Commons
:
Reconeixement-NoComercial-SenseObraDerivada 3.0 Espanya
Abstract
Objectives
This study aimed to compare the performance of a xenograft (XG) and a biomimetic synthetic graft (SG) in three-wall alveolar defects in minipigs by means of 3D computerised tomography and histology.
Materials and methods
Eight minipigs were used. A total of eight defects were created in the jaw of each animal, three of which were grafted with XGs, three with SGs, and two were left empty as a negative control. The allocation of the different grafts was randomised. Four animals were euthanised at 6 weeks and four at 12 weeks. The grafted volume was then measured by spiral computed tomography to assess volume preservation. Additionally, a histological analysis was performed in undecalcified samples by backscattered scanning electron microscopy and optical microscopy after Masson’s trichrome staining.
Results
A linear mixed-effects model was applied considering four fixed factors (bone graft type, regeneration time, anatomic position, and maxilla/mandible) and one random factor (animal). The SG exhibited significantly larger grafted volume (19%) than the XG. The anterior sites preserved better the grafted volume than the posterior ones. Finally, regeneration time had a positive effect on the grafted volume. Histological observations revealed excellent osseointegration and osteoconductive properties for both biomaterials. Some concavities found in the spheroidal morphologies of SGs were associated with osteoclastic resorption.
Conclusions
Both biomaterials met the requirements for bone grafting, i.e. biocompatibility, osseointegration, and osteoconduction. Granule morphology was identified as an important factor to ensure a good volume preservation.
Clinical relevance
Whereas both biomaterials showed excellent osteoconduction, SGs resulted in better volume preservation.
CitacióRaymond, S. [et al.]. Computed tomography and histological evaluation of xenogenic and biomimetic bone grafts in three-wall alveolar defects in minipigs. "Clinical oral investigations", 1 Maig 2021, p. 1-12.
ISSN1432-6981
Versió de l'editorhttps://link.springer.com/article/10.1007%2Fs00784-021-03956-y
Fitxers | Descripció | Mida | Format | Visualitza |
---|---|---|---|---|
Raymond2021_Art ... TomographyAndHistologi.pdf | 8,925Mb | Visualitza/Obre |