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dc.contributor.authorBueno García, Juan Manuel
dc.contributor.authorPalacios Higueras, Raquel
dc.contributor.authorGualda Manzano, Emilio José
dc.contributor.authorArtal Soriano, Pablo
dc.contributor.authorSkorsetz, Martin
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament d'Enginyeria Agroalimentària i Biotecnologia
dc.identifier.citationBueno, J. [et al.]. Multiphoton imaging microscopy at deeper layers with adaptive optics control of spherical aberration. "Journal of biomedical optics", 17 Juliol 2013, vol. 19, núm. 1, p. 0110071-0110077.
dc.description.abstractDespite the inherent confocality and optical sectioning capabilities of multiphoton microscopy, threedimensional (3-D) imaging of thick samples is limited by the specimen-induced aberrations. The combination of immersion objectives and sensorless adaptive optics (AO) techniques has been suggested to overcome this difficulty. However, a complex plane-by-plane correction of aberrations is required, and its performance depends on a set of image-based merit functions. We propose here an alternative approach to increase penetration depth in 3-D multiphoton microscopy imaging.
dc.description.sponsorshipThis work has been supported by “Ministerio de Ciencia e Innovación,” Spain (Grants FIS2010-14926 & Consolider Program SAUUL CSD2007-00013), “Fundación Séneca,” Murcia, Spain (Grant 04524/GERM/06), and EU-FEDER funds
dc.format.extent7 p.
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.subjectÀrees temàtiques de la UPC::Física::Física de partícules::Fotons
dc.subject.lcshMultiphoton processes
dc.subject.lcshFluorescence microscopy
dc.subject.otherMultiphoton microscopy
dc.subject.otherWavefront aberration
dc.subject.otherAdaptive optics
dc.subject.otherHuman ocular tissue
dc.titleMultiphoton imaging microscopy at deeper layers with adaptive optics control of spherical aberration
dc.subject.lemacMicroscòpia de fluorescència
dc.rights.accessOpen Access
dc.description.versionPostprint (published version)
local.citation.authorBueno, J.; Palacios, R.; Gualda Manzano, Emilio Jose; Artal Soriano, Pablo; Skorsetz, M.
local.citation.publicationNameJournal of biomedical optics

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