Multiphoton imaging microscopy at deeper layers with adaptive optics control of spherical aberration
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Despite the inherent confocality and optical sectioning capabilities of multiphoton microscopy, threedimensional (3-D) imaging of thick samples is limited by the specimen-induced aberrations. The combination of immersion objectives and sensorless adaptive optics (AO) techniques has been suggested to overcome this difficulty. However, a complex plane-by-plane correction of aberrations is required, and its performance depends on a set of image-based merit functions. We propose here an alternative approach to increase penetration depth in 3-D multiphoton microscopy imaging.
CitationBueno, J. [et al.]. Multiphoton imaging microscopy at deeper layers with adaptive optics control of spherical aberration. "Journal of biomedical optics", 17 Juliol 2013, vol. 19, núm. 1, p. 0110071-0110077.