Retinal cell imaging in myopic chickens using adaptive optics multiphoton microscopy
Visualitza/Obre
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hdl:2117/345877
Tipus de documentArticle
Data publicació2014-03-01
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Abstract
Abnormal eye growth induced by visual deprivation can modify
the structure and density of the retinal cells. We have used an adaptive
optics multiphoton microscope to image photoreceptors (PRs) and ganglion
cells (GCs) at different retinal locations in unstained retinas of chicken eyes
with about 10D of myopia and their normal-sighted fellow eyes. In all
samples, the local averaged inter-PR distance increased with eccentricity.
No significant differences in PR density were found between control and
myopic eyes. GC density declined in myopic eyes compared to control eyes
and the inter-cell distance increased. In normal eyes, the size of the GC cell
bodies increased approximately two-fold between the area centralis and the
peripheral retina. In myopic eyes, this trend was preserved but the GC
bodies were larger at each retinal location, compared to control eyes.
Obviously, GC morphology is changing when the retinal area is enlarged in
myopic eyes.
Descripció
This work has been supported by “Ministerio de Ciencia e Innovación”, Spain (grants
FIS2010-14926 and CSD2007-00013 SAUUL); Fundación Séneca (Region de Murcia,
Spain), grant 4524/GERM/06 and EU FEDER funds. The authors would like to thank Eva
Burkhardt from the Institute for Ophthalmic Research (Tuebingen, Germany) for helping in
the preparation of retinal tissues
CitacióBueno, J. [et al.]. Retinal cell imaging in myopic chickens using adaptive optics multiphoton microscopy. "Biomedical optics express", 1 Març 2014, vol. 5, núm. 3, p. 664-674.
ISSN2156-7085
Versió de l'editorhttp://www.opticsinfobase.org/boe/abstract.cfm?uri=boe-5-3-664
Col·leccions
Fitxers | Descripció | Mida | Format | Visualitza |
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2014_Bio_Opt_Exp.pdf | 1,693Mb | Visualitza/Obre |