Retinal cell imaging in myopic chickens using adaptive optics multiphoton microscopy
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Abnormal eye growth induced by visual deprivation can modify the structure and density of the retinal cells. We have used an adaptive optics multiphoton microscope to image photoreceptors (PRs) and ganglion cells (GCs) at different retinal locations in unstained retinas of chicken eyes with about 10D of myopia and their normal-sighted fellow eyes. In all samples, the local averaged inter-PR distance increased with eccentricity. No significant differences in PR density were found between control and myopic eyes. GC density declined in myopic eyes compared to control eyes and the inter-cell distance increased. In normal eyes, the size of the GC cell bodies increased approximately two-fold between the area centralis and the peripheral retina. In myopic eyes, this trend was preserved but the GC bodies were larger at each retinal location, compared to control eyes. Obviously, GC morphology is changing when the retinal area is enlarged in myopic eyes.
This work has been supported by “Ministerio de Ciencia e Innovación”, Spain (grants FIS2010-14926 and CSD2007-00013 SAUUL); Fundación Séneca (Region de Murcia, Spain), grant 4524/GERM/06 and EU FEDER funds. The authors would like to thank Eva Burkhardt from the Institute for Ophthalmic Research (Tuebingen, Germany) for helping in the preparation of retinal tissues
CitationBueno, J. [et al.]. Retinal cell imaging in myopic chickens using adaptive optics multiphoton microscopy. "Biomedical optics express", 1 Març 2014, vol. 5, núm. 3, p. 664-674.