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Wavefront optimized nonlinear microscopy of ex vivo human retinas
dc.contributor.author | Gualda Manzano, Emilio José |
dc.contributor.author | Artal Soriano, Pablo |
dc.contributor.author | Bueno García, Juan Manuel |
dc.contributor.other | Universitat Politècnica de Catalunya. Departament d'Enginyeria Agroalimentària i Biotecnologia |
dc.date.accessioned | 2021-04-27T13:50:59Z |
dc.date.issued | 2010 |
dc.identifier.citation | Gualda Manzano, E.J.; Artal Soriano, P.; Bueno, J. Wavefront optimized nonlinear microscopy of ex vivo human retinas. "Journal of biomedical optics", 2010, vol. 15, núm. 2, p. 1-7. |
dc.identifier.issn | 1083-3668 |
dc.identifier.uri | http://hdl.handle.net/2117/344598 |
dc.description.abstract | A multiphoton microscope incorporating a HartmannShack HS wavefront sensor to control the ultrafast laser beam’s wavefront aberrations has been developed. This instrument allowed us to investigate the impact of the laser beam aberrations on twophoton autofluorescence imaging of human retinal tissues. We demonstrated that nonlinear microscopy images are improved when laser beam aberrations are minimized by realigning the laser system cavity while wavefront controlling. |
dc.description.sponsorship | This work has been supported by the Ministerio de Educación y Ciencia, Spain Grant Nos. FIS2007-64765 and Consolider SAUUL CSD2007-00033, and Fundación Séneca, Murcia, Spain Grant 04524/GERM/06. The authors thank J. M.Marín, MD, PhD, from the Hospital Universitario “Virgen de la Arrixaca,” Murcia, Spain, for providing us with the ocular tissue samples |
dc.format.extent | 7 p. |
dc.language.iso | eng |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Spain |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ |
dc.subject | Àrees temàtiques de la UPC::Física::Física de partícules::Fotons |
dc.subject.lcsh | Microscopy |
dc.subject.other | Multiphoton microscopy |
dc.subject.other | Two-photon fluorescence |
dc.subject.other | Wavefront aberration |
dc.subject.other | Human retina |
dc.title | Wavefront optimized nonlinear microscopy of ex vivo human retinas |
dc.type | Article |
dc.subject.lemac | Microscòpia |
dc.identifier.doi | 10.1117/1.3369001 |
dc.relation.publisherversion | https://www.spiedigitallibrary.org/journals/journal-of-biomedical-optics/volume-15/issue-02/026007/Wavefront-optimized-nonlinear-microscopy-of-iex-vivoi-human-retinas/10.1117/1.3369001.full?SSO=1 |
dc.rights.access | Restricted access - publisher's policy |
local.identifier.drac | 30363199 |
dc.description.version | Postprint (published version) |
dc.date.lift | 10000-01-01 |
local.citation.author | Gualda Manzano, Emilio Jose; Artal Soriano, Pablo; Bueno, J. |
local.citation.publicationName | Journal of biomedical optics |
local.citation.volume | 15 |
local.citation.number | 2 |
local.citation.startingPage | 1 |
local.citation.endingPage | 7 |
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