Self-regulation of cell population in microbial consortia
Realitzat a/ambUniversità degli Studi di Napoli Federico II
Tipus de documentProjecte Final de Màster Oficial
Data2020-07-09
Condicions d'accésAccés obert
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Reconeixement-NoComercial-SenseObraDerivada 3.0 Espanya
Abstract
In recent years, multicellular applications have been increased in the field of synthetic biology. However, this has led to the appearance of a new problem; ensure the stable co-existence of the populations in microbial consortia. As different cell types have different growth conditions,
they tend to compete with each other for resources, leading to the disappearance of one of the populations. This issue has been studied in recent years by multiple research groups. However, in all these previous works the final model proposed either needed some external inputs or implied a mutual dependence between the two populations. In thesis we present a novel approach to the problem of stable coexistence of different cell populations in microbial consortia, proposing a design in which the cells are able of self-regulating their relative number in the consortium. This is achieved by embedding a bistable memory mechanism, a genetic toggle switch, inside each cell, so that the current internal state defines the population the cell belongs to. On one hand, taking as reference previous works, the mathematical model of the system, as well as a simplified version of it, will be developed. On the other hand, the numerical validation of the model will be performed
including an analysis of the parameters role in the system, a study of the capability of tuning the final ratio and a robustness analysis to cell heterogeneity
TitulacióMÀSTER UNIVERSITARI EN ENGINYERIA INDUSTRIAL (Pla 2014)
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final-master-thesis.pdf | 2,022Mb | Visualitza/Obre | ||
code.zip | 11,68Kb | application/zip | Visualitza/Obre |