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dc.contributor.authorIvanova, Kristina Dimitrova
dc.contributor.authorAsenova Ivanova, Aleksandra
dc.contributor.authorRamon Portés, Eva
dc.contributor.authorHoyo Pérez, Javier
dc.contributor.authorSanchez-Gomez, Susana
dc.contributor.authorTzanov, Tzanko
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament d'Enginyeria Química
dc.contributor.otherUniversitat Politècnica de Catalunya. Doctorat en Polímers i Biopolímers
dc.identifier.citationIvanova, K. [et al.]. Antibody-enabled antimicrobial nanocapsules for selective elimination of Staphylococcus aureus. "ACS applied materials and interfaces", 16 Juliol 2020, vol. 12, núm. 32, p. 35918-35927.
dc.description.abstractTargeted bactericide nanosystems hold significant promise to improve the efficacy of existing antimicrobials for treatment of severe bacterial infections, minimizing the side effects and lowering the risk of antibiotic resistance occurrence. In this work, we developed antibody functionalized nanocapsules (NCs) containing antibacterial essential oil (EO) for selective and effective eradication of Staphylococcus aureus. Antibacterial EO NCs were produced via self-assembling nanoencapsulation in the plant-derived protein zein. The obtained EO NCs were decorated with aminocellulose to provide more reactive surface groups for carboxyl-to-amine immobilization of a specific against S. aureus antibody. The antibody-enabled EO NCs (Ab@EO NCs) demonstrated 2-fold higher bactericidal efficacy against the targeted bacterium compared to the pristine EO NCs at the same concentrations. The improved antibacterial effect of the Ab@EO NCs towards S. aureus was also confirmed in a real time assay by monitoring bacterial cells elimination using a quartz crystal microbalance. Furthermore, the Ab@EO selectively decreased the load and changed the cell morphology of the targeted S. aureus in a mixed inoculum with non-targeted P. aeruginosa. Applying the nanoformulated actives to an in vitro co-culture model of the bacteria and skin fibroblasts resulted in suppression of S. aureus growth, while preserving the human cells viability. The novel antibody-enabled antibacterial NCs showed potential to improve the treatment efficacy of staphylococcal infections, minimally affecting the beneficial microbiome and human cells.
dc.format.extent10 p.
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.subjectÀrees temàtiques de la UPC::Enginyeria química
dc.subject.lcshStaphylococcus aureus
dc.subject.otherStaphylococcus aureus
dc.subject.otherAntibiotic resistance
dc.subject.otherEssential oil
dc.subject.otherZein nanocapsules
dc.subject.otherTargeting antibody
dc.subject.otherSelective antibacterial activity
dc.titleAntibody-enabled antimicrobial nanocapsules for selective elimination of Staphylococcus aureus
dc.subject.lemacEstafilococs daurats
dc.subject.lemacMalalties bacterianes
dc.contributor.groupUniversitat Politècnica de Catalunya. GBMI - Grup de Biotecnologia Molecular i Industrial
dc.description.peerreviewedPeer Reviewed
dc.rights.accessRestricted access - publisher's policy
dc.description.versionPostprint (author's final draft)
local.citation.authorIvanova, K.; Asenova, A.; Ramon, E.; Hoyo, J.; Sanchez-Gomez, S.; Tzanov, T.
local.citation.publicationNameACS applied materials and interfaces

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