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dc.contributor.authorGellings, Esther
dc.contributor.authorCogdell, Richard J.
dc.contributor.authorvan Hulst, Niek F.
dc.date.accessioned2020-08-21T07:00:03Z
dc.date.available2021-03-05T01:28:18Z
dc.date.issued2020-03-06
dc.identifier.citationGellings, E.; Cogdell, R.J.; Van Hulst, N.F. Room Temperature Excitation-Emission Spectra of Single LH2 Complexes Show Remarkably Little Variation. "The Journal of Physical Chemistry Letters", 6 Març 2020, vol. 11, núm. 7, p. 2430-2435.
dc.identifier.urihttp://hdl.handle.net/2117/328215
dc.description.abstractExcitation spectroscopy gives direct insight into the excited state manifold, energy transfer, transient intermediates, vibrations, and so on. Unfortunately, excitation spectroscopy of single molecules under ambient conditions has remained challenging. Here we present excitation spectra alongside emission spectra of the same individual light-harvesting complex LH2 of the purple bacteria Rps. acidophila. The acquisition of both the excited and ground state spectra allows us to quantify disorder and interband correlations, which are key variables for the interpretation of observed long-lasting coherences. We have overcome the low photostability and small fluorescence quantum yield that are inherent to many biologically relevant systems by combining single-molecule Fourier transform spectroscopy, low excitation intensities, and effective data analysis. We find that LH2 complexes show little spectral variation (130–170 cm–1), that their two absorption bands (B800–B850) are uncorrelated, and that the Stokes shift is not constant. The low amount of spectral disorder underlines the protective role of the protein scaffold, benefiting the efficient energy transport throughout the light-harvesting membrane.
dc.format.extent6 p.
dc.language.isoeng
dc.publisherACS
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectÀrees temàtiques de la UPC::Física
dc.subject.lcshSpectrum analysis
dc.subject.otherspectroscopy
dc.titleRoom Temperature Excitation-Emission Spectra of Single LH2 Complexes Show Remarkably Little Variation
dc.typeArticle
dc.subject.lemacAnàlisi espectral
dc.identifier.doi10.1021/acs.jpclett.0c00375
dc.description.peerreviewedPeer Reviewed
dc.relation.publisherversionhttps://pubs.acs.org/doi/10.1021/acs.jpclett.0c00375
dc.rights.accessOpen Access
dc.description.versionPostprint (author's final draft)
dc.relation.projectidSEV-2014-0124
dc.relation.projectid670949-LightNet
dc.relation.projectidSEV-2015-0522
dc.relation.projectidFIS2015-69258-P
dc.relation.projectid2017SGR1369
local.citation.publicationNameThe Journal of Physical Chemistry Letters
local.citation.volume11
local.citation.number7
local.citation.startingPage2430
local.citation.endingPage2435


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Attribution-NonCommercial-NoDerivs 3.0 Spain
Except where otherwise noted, content on this work is licensed under a Creative Commons license : Attribution-NonCommercial-NoDerivs 3.0 Spain