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dc.contributor.authorMas Moruno, Carlos
dc.contributor.authorGarrido, Beatriz
dc.contributor.authorRodríguez Rius, Daniel
dc.contributor.authorRupérez de Gracia, Elisa
dc.contributor.authorGil Mur, Francisco Javier
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica
dc.identifier.citationMas-Moruno, C. [et al.]. Biofunctionalization strategies on tantalum-based materials for osseointegrative applications. "J Mater Sci: Mater Med", 11 Febrer 2015.
dc.description.abstractThe use of tantalum as biomaterial for orthopedic applications is gaining considerable attention in the clinical practice because it presents an excellent chemical stability, body fluid resistance, biocompatibility, and it is more osteoconductive than titanium or cobalt-chromium alloys. Nonetheless, metallic biomaterials are commonly bioinert and may not provide fast and long-lasting interactions with surrounding tissues. The use of short cell adhesive peptides derived from the extracellular matrix has shown to improve cell adhesion and accelerate the implant’s biointegration in vivo. However, this strategy has been rarely applied to tantalum materials. In this work, we have studied two immobilization strategies (physical adsorption and covalent binding via silanization) to functionalize tantalum surfaces with a cell adhesive RGD peptide. Surfaces were used untreated or activated with either HNO3 or UV/ozone treatments. The process of biofunctionalization was characterized by means of physicochemical and biological methods. Physisorption of the RGD peptide on control and HNO3-treated tantalum surfaces significantly enhanced the attachment and spreading of osteoblast-like cells; however, no effect on cell adhesion was observed in ozone-treated samples. This effect was attributed to the inefficient binding of the peptide on these highly hydrophilic surfaces, as evidenced by contact angle measurements and X-ray photoelectron spectroscopy. In contrast, activation of tantalum with UV/ozone proved to be the most efficient method to support silanization and subsequent peptide attachment, displaying the highest values of cell adhesion. This study demonstrates that both physical adsorption and silanization are feasible methods to immobilize peptides onto tantalum-based materials, providing them with superior bioactivity.
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.subjectÀrees temàtiques de la UPC::Enginyeria dels materials
dc.subject.lcshBiomedical materials
dc.titleBiofunctionalization strategies on tantalum-based materials for osseointegrative applications
dc.subject.lemacMaterials biomèdics
dc.subject.lemacTàntal (Química inorgànica)
dc.contributor.groupUniversitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits
dc.description.peerreviewedPeer Reviewed
dc.rights.accessOpen Access
dc.description.versionPostprint (published version)
dc.relation.projectidinfo:eu-repo/grantAgreement/EC/FP7/321985/EU/Development of new biofunctionalized materials for application in regenerative medicine/BIOMAT4BIOMED
upcommons.citation.authorMas-Moruno, C.; Garrido, B.; Rodriguez, D.; Rupérez de Gracia, E.; Gil, F.J.
upcommons.citation.publicationNameJ Mater Sci: Mater Med

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