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dc.contributor.authorMorató Farreras, Jordi
dc.contributor.authorCodony Iglesias, Francesc
dc.contributor.authorSánchez Lorente, Olga
dc.contributor.authorMartín Pérez, Leonardo
dc.contributor.authorGarcía Serrano, Joan
dc.contributor.authorMas Herrero, Jordi
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament d'Òptica i Optometria
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament d'Enginyeria Hidràulica, Marítima i Ambiental
dc.identifier.citationMorato, J. [et al.]. Key design factors affecting microbial community composition and pathogenic organism removal in horizontal subsurface flow constructed wetlands. "Science of the total environment", 15 Maig 2014, vol. 481, p. 81-89.
dc.description.abstractConstructed wetlands constitute an interesting option for wastewater reuse since high concentrations of contaminants and pathogenic microorganisms can be removed with these natural treatment systems. In this work, the role of key design factors which could affect microbial removal and wetland performance, such as granular media, water depth and season effect was evaluated in a pilot system consisting of eight parallel horizontal subsurface flow (HSSF) constructed wetlands treating urban wastewater from Les Franqueses del Valles (Barcelona, Spain). Gravel biofilm as well as influent and effluent water samples of these systems were taken in order to detect the presence of bacterial indicators such as total coliforms (TC), Escherichia coil, fecal enterococci (FE), Clostridium perfringens, and other microbial groups such as Pseudomonas and Aeromonas. The overall microbial inactivation ratio ranged between 1.4 and 2.9 log-units for heterotrophic plate counts (HPC), from 1.2 to 2.2 log units for total coliforms (TC) and from 1.4 to 2.3 log units for E. coli. The presence of fine granulometry strongly influenced the removal of all the bacterial groups analyzed. This effect was significant for TC (p = 0.009), E. coli (p = 0.004), and FE (p = 0.012). Shallow HSSF constructed wetlands were more effective for removing Clostridium spores (p = 0.039), and were also more efficient for removing TC (p = 0.011) and E. coil (p = 0.013) when fine granulometry was used. On the other hand, changes in the total bacterial community from gravel biofilm were examined by using denaturing gradient gel electrophoresis (DGGE) and sequencing of polymerase chain reaction (PCR)-amplified fragments of the 16S rRNA gene recovered from DGGE bands. Cluster analysis of the DGGE banding pattern from the different wetlands showed that microbial assemblages separated according to water depth, and sequences of different phylogenetic groups, such as Alpha, Beta and Delta-Proteobacteria, Nitrospirae, Bacteroidetes, Acidobacteria, Firmicutes, Synergistetes and Deferribacteres could be retrieved from DGGE bands. (C) 2014 Elsevier B.V. All rights reserved.
dc.format.extent9 p.
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.subjectÀrees temàtiques de la UPC::Desenvolupament humà i sostenible::Política i gestió ambiental
dc.subjectÀrees temàtiques de la UPC::Enginyeria agroalimentària::Ciències de la terra i de la vida::Microbiologia
dc.subject.lcshConstructed wetlands
dc.subject.lcshWaste disposal in the ground
dc.subject.lcshMicrobial diversity
dc.subject.otherConstructed wetlands
dc.subject.otherHorizontal subsurface flow
dc.subject.otherDesign factors
dc.subject.otherMunicipal wastewater treatment
dc.subject.otherGravel biofilm examination
dc.subject.otherREED BEDS
dc.titleKey design factors affecting microbial community composition and pathogenic organism removal in horizontal subsurface flow constructed wetlands
dc.subject.lemacAigües residuals -- Depuració
dc.contributor.groupUniversitat Politècnica de Catalunya. SUMMLab - Sustainability Measurement and Modeling Lab
dc.contributor.groupUniversitat Politècnica de Catalunya. TMAS (T+) - Toxicologia i Microbiologia Ambiental i Sanitària
dc.contributor.groupUniversitat Politècnica de Catalunya. GEMMA - Grup d'Enginyeria i Microbiologia del Medi Ambient
dc.rights.accessRestricted access - publisher's policy
dc.description.versionPostprint (published version)
local.citation.authorMorato, J.; Codony, F.; Sánchez, O.; Martín, L.; Garcia, J.; Mas, J.
local.citation.publicationNameScience of the total environment

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