Show simple item record

dc.contributor.authorTam, Johnny
dc.contributor.authorCordier, Guillaume Alan
dc.contributor.authorBorbely, Joseph Steven
dc.contributor.authorSandoval Alvarez, Angel
dc.contributor.authorLakadamyali, Melike
dc.contributor.otherUniversitat Politècnica de Catalunya. Institut de Ciències Fotòniques
dc.date.accessioned2014-12-04T15:46:00Z
dc.date.available2014-12-04T15:46:00Z
dc.date.issued2014-07-07
dc.identifier.citationTam, Johnny [et al.]. Cross-Talk-Free Multi-Color STORM Imaging Using a Single Fluorophore. "PLOS ONE", 7 Juliol 2014, vol. 9, núm. 7.
dc.identifier.urihttp://hdl.handle.net/2117/24928
dc.description.abstractMulti-color stochastic optical reconstruction microscopy (STORM) is routinely performed; however, the various approaches for achieving multiple colors have important caveats. Color cross-talk, limited availability of spectrally distinct fluorophores with optimal brightness and duty cycle, incompatibility of imaging buffers for different fluorophores, and chromatic aberrations impact the spatial resolution and ultimately the number of colors that can be achieved. We overcome these complexities and develop a simple approach for multi-color STORM imaging using a single fluorophore and sequential labelling. In addition, we present a simple and versatile method to locate the same region of interest on different days and even on different microscopes. In combination, these approaches enable cross-talk-free multi-color imaging of sub-cellular structures.
dc.format.extent7
dc.language.isoeng
dc.publisherPLOS
dc.subjectÀrees temàtiques de la UPC::Física
dc.subject.lcshOptical Imaging
dc.subject.otherSTORM
dc.titleCross-Talk-Free Multi-Color STORM Imaging Using a Single Fluorophore
dc.typeArticle
dc.subject.lemacEscàner, Sistemes d'
dc.identifier.doi10.1371/journal.pone.0101772
dc.description.peerreviewedPeer Reviewed
dc.relation.publisherversionhttp://www.plosone.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0101772&representation=PDF
dc.rights.accessOpen Access
dc.description.versionPostprint (published version)
dc.relation.projectidinfo:eu-repo/grantAgreement/EC/FP7/337191/EU/On the move: Motor-cargo and motor-microtubule interactions studied with quantitative, high spatio-temporal resolution microscopy in vivo/MOTORS
upcommons.citation.authorTam, Johnny; Cordier, Guillaume Alan; Borbely, Joseph Steven; Sandoval Alvarez, Angel; Lakadamyali, Melike
upcommons.citation.publishedtrue
upcommons.citation.publicationNamePLOS ONE
upcommons.citation.volume9
upcommons.citation.number7
upcommons.citation.startingPagee101772
dc.identifier.pmid25000286


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

All rights reserved. This work is protected by the corresponding intellectual and industrial property rights. Without prejudice to any existing legal exemptions, reproduction, distribution, public communication or transformation of this work are prohibited without permission of the copyright holder