Cross-Talk-Free Multi-Color STORM Imaging Using a Single Fluorophore
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European Commission's projectMOTORS - On the move: Motor-cargo and motor-microtubule interactions studied with quantitative, high spatio-temporal resolution microscopy in vivo (EC-FP7-337191)
Multi-color stochastic optical reconstruction microscopy (STORM) is routinely performed; however, the various approaches for achieving multiple colors have important caveats. Color cross-talk, limited availability of spectrally distinct fluorophores with optimal brightness and duty cycle, incompatibility of imaging buffers for different fluorophores, and chromatic aberrations impact the spatial resolution and ultimately the number of colors that can be achieved. We overcome these complexities and develop a simple approach for multi-color STORM imaging using a single fluorophore and sequential labelling. In addition, we present a simple and versatile method to locate the same region of interest on different days and even on different microscopes. In combination, these approaches enable cross-talk-free multi-color imaging of sub-cellular structures.
CitationTam, Johnny [et al.]. Cross-Talk-Free Multi-Color STORM Imaging Using a Single Fluorophore. "PLOS ONE", 7 Juliol 2014, vol. 9, núm. 7.