Mostra el registre d'ítem simple

dc.contributor.authorPrieto Simón, Beatriz
dc.contributor.authorMacanás de Benito, Jorge
dc.contributor.authorMuñoz Tapia, Maria
dc.contributor.authorFábregas Martínez, Esteve
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament d'Enginyeria Química
dc.date.accessioned2014-10-20T12:44:52Z
dc.date.created2007-03-30
dc.date.issued2007-03-30
dc.identifier.citationPrieto-Simón, B. [et al.]. Evaluation of different mediator-modified screen-printed electrodes used in a flow system as amperometric sensors for NADH. "Talanta", 30 Març 2007, vol. 71, núm. 5, p. 2102-2107.
dc.identifier.issn0039-9140
dc.identifier.urihttp://hdl.handle.net/2117/24433
dc.description.abstractThis work presents a comparative study between two different methods for the preparation of mediator-modified screen-printed electrodes, to be used as detectors in a reliable flow injection system for the determination of the nicotinamide adenine dinucleotide (NADH) coenzyme. The best strategy was selected for the final development of compact biosensors based on dehydrogenase enzymes. For the first immobilisation strategy, different redox mediators were electropolymerised onto the SPE surface. The second immobilisation strategy was carried out using polysulfone–graphite composites, which were deposited by screen-printing technology onto the screen-printed electrode (SPE) surface. Both methods achieved an effective and reliable incorporation of redox mediators to the SPE configuration. Finally, a flow system for ammonium determination was developed using a glutamate dehydrogenase (GlDH)-Meldola’s Blue (MB)-polysulfone-composite film-based biosensor. The stability of the redox mediators inside the composite films as well as the negligible fouling effect observed on the electrode surface improve the repeatability and reproducibility of the sensors, important features for continuous analysis in flow systems. Furthermore, the optimised bio/sensors, incorporated in a flow injection system, showed good sensitivities and short response times. Such a good analytical performance together with the simple and fast sensor construction are interesting characteristics to consider the polysulfone-composite films as attractive electrochemical transducer materials for the development of new dehydrogenase-based SPEs.
dc.format.extent6 p.
dc.language.isoeng
dc.subjectÀrees temàtiques de la UPC::Enginyeria química
dc.subject.lcshSulfones
dc.subject.lcshThermoplastics
dc.subject.lcshPolymers
dc.subject.lcshSerigraphy
dc.subject.lcshFlow injection analysis
dc.subject.otherSPE
dc.subject.otherFlow injection analysis
dc.subject.otherPolysulfone
dc.subject.otherRedox mediators
dc.titleEvaluation of different mediator-modified screen-printed electrodes used in a flow system as amperometric sensors for NADH
dc.typeArticle
dc.subject.lemacPolisulfurs
dc.subject.lemacPolímers
dc.subject.lemacTermoplàstics
dc.subject.lemacAnàlisi per injecció en flux
dc.contributor.groupUniversitat Politècnica de Catalunya. POLQUITEX - Materials Polimérics i Química Téxtil
dc.identifier.doi10.1016/j.talanta.2006.09.022
dc.rights.accessRestricted access - publisher's policy
local.identifier.drac14908223
dc.description.versionPostprint (published version)
dc.date.lift10000-01-01
local.citation.authorPrieto-Simón, B.; Macanas, J.; Muñoz, M.; Fábregas, E.
local.citation.publicationNameTalanta
local.citation.volume71
local.citation.number5
local.citation.startingPage2102
local.citation.endingPage2107


Fitxers d'aquest items

Imatge en miniatura

Aquest ítem apareix a les col·leccions següents

Mostra el registre d'ítem simple