Advanced fluorescence imaging and biophysics

Recent Submissions

  • DNA Origami offers a versatile method for quantifying protein copy‐number in superresolution 

    Zanacchi, Francesca Cella; Manzo, Carlo; Sandoval Alvarez, Angel; Derr, Nathan D.; Garcia-Parajo, Maria F.; Lakadamyali, Melike (Nature, 2017-06-26)
    Article
    Open Access
    Single-molecule-based super-resolution microscopy offers researchers a unique opportunity to quantify protein copy number with nanoscale resolution. However, while fluorescent proteins have been characterized for quantitative ...

  • Super-resolution microscopy: going live and going fast 

    Lakadamyali, Melike (Wiley, 2014-03-17)
    Article
    Open Access
    Super-resolution microscopy is increasingly becoming an important tool for biological research, providing valuable information at the nanometer length scales inside cells and tissues. In the past decade numerous ...

  • Human N-Methyl-D-aspartate receptor antibodies alter memory and behavior in mice 

    Planagumà, Jesús; Leypoldt, Frank; Mannara, Francesco; Gutiérrez-Cuesta, Javier; Martín-García, Elena; Aguilar, Esther; Titulaer, Maarten J.; Jain, Ankit; Balice-Gordon, Rita; Lakadamyali, Melike; Graus, Francesc; Maldonado, Rafael; Dalmau, Josep (Oxford, 2015-01)
    Article
    Open Access
    Anti-N-methyl D-aspartate receptor (NMDAR) encephalitis is a severe neuropsychiatric disorder that associates with prominent memory and behavioural deficits. Patients’ antibodies react with the N-terminal domain of the ...

  • Super-resolution imaging with stochastic single molecule localization: concepts, technical developments, and biological applications 

    Oddone, Anna; Verdeny Vilanova, Ione; Tam, Johnny; Lakadamyali, Melike (Wiley, 2014-07-01)
    Article
    Open Access
    Light microscopy has undergone a revolution with the advent of super-resolution microscopy methods that can surpass the diffraction limit. These methods have generated much enthusiasm, in particular with regards to the new ...

  • Quantitative super-resolution microscopy: pitfalls and strategies for image analysis 

    Durisic, Nela; Laparra Cuervo, Lara; Lakadamyali, Melike (ScienceDirect, 2014-06-01)
    Article
    Open Access
    Super-resolution microscopy is an enabling technology that allows biologists to visualize cellular structures at nanometer length scales using far-field optics. To break the diffraction barrier, it is necessary to leverage ...

  • Single molecule evaluation of fluorescent protein photoactivation efficiency using an in vivo nanotemplate 

    Durisic, Nela; Laparra-Cuervo, Lara; Sandoval-Álvarez, Ángel; Borbely, Joseph Steven; Lakadamyali, Melike (Nature, 2014-01-05)
    Article
    Open Access
    Photoswitchable fluorescent probes are central to localization-based super-resolution microscopy. Among these probes, fluorescent proteins are appealing because they are genetically encoded. Moreover, the ability to achieve ...

  • Human N-methyl D-aspartate receptor antibodies alter memory and behaviour in mice 

    Planagumà, Jesús; Leypoldt, Frank; Mannara, Francesco; Gutiérrez-Cuesta, Javier; Martín-García, Elena; Aguilar, Esther; Titulaer, Maarten J.; Petit-Pedrol, Mar; Jain, Ankit; Balice-Gordon, Rita; Lakadamyali, Melike; Graus, Francesc; Maldonado, Rafael; Dalmau, Josep (OUP, 2015-01-09)
    Article
    Restricted access - publisher's policy
    Anti-N-methyl D-aspartate receptor (NMDAR) encephalitis is a severe neuropsychiatric disorder that associates with prominent memory and behavioural deficits. Patients’ antibodies react with the N-terminal domain of the ...

  • Cross-Talk-Free Multi-Color STORM Imaging Using a Single Fluorophore 

    Tam, Johnny; Cordier, Guillaume Alan; Borbely, Joseph Steven; Sandoval Alvarez, Angel; Lakadamyali, Melike (PLOS, 2014-07-07)
    Article
    Open Access
    Multi-color stochastic optical reconstruction microscopy (STORM) is routinely performed; however, the various approaches for achieving multiple colors have important caveats. Color cross-talk, limited availability of ...