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  • PLANT: A Method for Detecting Changes of Slope in Noisy Trajectories 

    Sosa-Costa, Alberto; Piechocka, Izabela K.; Gardini, Lucia; Pavone, Francesco S.; Capitanio, Marco; Garcia-Parajo, Maria F.; Manzo, Carlo (ScienceDirect, 2018-05-08)
    Article
    Open Access
    Time traces obtained from a variety of biophysical experiments contain valuable information on underlying processes occurring at the molecular level. Accurate quantification of these data can help explain the details of ...

  • Optical Antenna-Based Fluorescence Correlation Spectroscopy to Probe the Nanoscale Dynamics of Biological Membranes 

    Winkler, Pamina M.; Regmi, Raju; Flauraud, Valentin; Brugger, Jürgen; Rigneault, Hervé; Wenger, Jérôme; García-Parajo, María F. (ACS, 2017-12-14)
    Article
    Open Access
    The plasma membrane of living cells is compartmentalized at multiple spatial scales ranging from the nano- to the mesoscale. This nonrandom organization is crucial for a large number of cellular functions. At the nanoscale, ...

  • TANGO1 builds a machine for collagen export by recruiting and spatially organizing COPII, tethers and membranes 

    Raote, Ishier; Ortega-Bellido, María; Santos, António J M; Foresti, Ombretta; Zhang, Chong; Garcia-Parajo, Maria F; Campelo, Felix; Malhotra, Vivek (eLife Sciences Publications, 2018-03-07)
    Article
    Open Access
    Collagen export from the endoplasmic reticulum (ER) requires TANGO1, COPII coats, and retrograde fusion of ERGIC membranes. How do these components come together to produce a transport carrier commensurate with the bulky ...

  • Planar Optical Nanoantennas Resolve Cholesterol-Dependent Nanoscale Heterogeneities in the Plasma Membrane of Living Cells 

    Regmi, Raju; Winkler, Pamina M.; Flauraud, Valentin; Borgman, Kyra J. E.; Manzo, Carlo; Brugger, Jürgen; Rigneault, Hervé; Wenger, Jérôme; García-Parajo, María (ACS, 2017-09-19)
    Article
    Open Access
    Optical nanoantennas can efficiently confine light into nanoscopic hotspots, enabling single-molecule detection sensitivity at biological relevant conditions. This innovative approach to breach the diffraction limit offers ...

  • Transient Nanoscopic Phase Separation in Biological Lipid Membranes Resolved by Planar Plasmonic Antennas 

    Winkler, Pamina M.; Regmi, Raju; Flauraud, Valentin; Brugger, Jürgen; Rigneault, Hervé; Wenger, Jérôme; García-Parajo, María F. (ACS, 2017-07-11)
    Article
    Open Access
    Nanoscale membrane assemblies of sphingolipids, cholesterol, and certain proteins, also known as lipid rafts, play a crucial role in facilitating a broad range of important cell functions. Whereas on living cell membranes ...

  • Sphingolipid metabolic flow controls phosphoinositide turnover at the trans Golgi network 

    Capasso, Serena; Sticco, Lucia; Rizzo, Riccardo; Pirozzi, Marinella; Russo, Domenico; Dathan, Nina A.; Campelo, Felix; Galen, Josse van; Hölttä-Vuori, Maarit; Turacchio, Gabriele; Hausser, Angelika; Malhotra, Vivek; Riezman, Isabelle; Riezman, Howard; Ikonen, Elina; Luberto, Chiara; Parashuraman, Seetharaman; Luini, Alberto; Angelo, Giovanni D (Embo press, 2017-04-10)
    Article
    Open Access
    Sphingolipids are membrane lipids, which are globally required for eukaryotic life. Sphingolipid composition varies among endomembranes with pre- and post-Golgi compartments being poor and rich in sphingolipids, respectively. ...

  • Sphingomyelin metabolism controls the shape and function of the Golgi cisternae 

    Campelo, Felix; Galen, Josse van; Turacchio, Gabriele; Parashuraman, Seetharaman; Kozlov, Michael M.; Garcia-Parajo, Maria F.; Malhotra, Vivek (eLife Sciences Publications, 2017-05-13)
    Article
    Open Access
    The flat Golgi cisterna is a highly conserved feature of eukaryotic cells, but how is this morphology achieved and is it related to its function in cargo sorting and export? A physical model of cisterna morphology led us ...

  • In-plane plasmonic antenna arrays with surface nanogaps for giant fluorescence enhancement 

    Flauraud, Valentin; Regmi, Raju; Winkler, Pamina Martina; Alexander, Duncan T. L.; Rigneault, Herve; Hulst, Niek F. van; Garcia-Parajo, Maria F.; Wenger, Jerome; Brugger, Juergen (ACS, 2017-02-10)
    Article
    Open Access
    Optical nanoantennas have a great potential for enhancing light-matter interactions at the nanometer scale, yet fabrication accuracy and lack of scalability currently limit ultimate antenna performance and applications. ...

  • Roadmap on biosensing and photonics with advanced nano-optical methods 

    Fabrizio, Enzo Di; Schlücker, Sebastian; Wenger, Jérôme; Regmi, Raju; Rigneault, Hervé; Calafiore, Giuseppe; West, Melanie; Cabrini, Stefano; Fleischer, Monika; Hulst, Niek F van; Garcia-Parajo, Maria F; Pucci, Annemarie; Cojoc, Dan; Hauser, Charlotte A E; Ni, Ming (IOP, 2016-05-10)
    Article
    Open Access
    This roadmap, through the contributions of ten groups worldwide, contains different techniques, methods and materials devoted to sensing in nanomedicine. Optics is used in different ways in the detection schemes. Raman, ...

  • Weak Ergodicity Breaking of Receptor Motion in Living Cells Stemming from Random Diffusivity 

    Manzo, Carlo; Torreno-Pina, Juan A.; Massignan, Pietro; Lapeyre, Gerald J.; Lewenstein, Maciej; García Parajo, María F. (APS Physics, 2015-02-25)
    Article
    Open Access
    Molecular transport in living systems regulates numerous processes underlying biological function. Although many cellular components exhibit anomalous diffusion, only recently has the subdiffusive motion been associated ...

  • Nanoclustering as a dominant feature of plasma membrane organization 

    García-Parajo, María F.; Cambi, Alessandra; Torreño-Piña, Juan A.; Thomoson, Nancy; Jacobson, Ken (The Company of Biologists, 2014-12-01)
    Article
    Open Access
    Early studies have revealed that some mammalian plasma membrane proteins exist in small nanoclusters. The advent of super-resolution microscopy has corroborated and extended this picture, and led to the suggestion that ...

  • Priming by Chemokines Restricts Lateral Mobility of the Adhesion Receptor LFA-1 and Restores Adhesion to ICAM-1 Nano-Aggregates on Human Mature Dendritic Cells 

    Borgman, Kyra J.; Zanten, Thomas S. van; Manzo, Carlo; Cabezón, Raquel; Cambi, Alessandra; Benítez-Ribas, Daniel; Garcia-Parajo, Maria F. (PLOS, 2014-06-19)
    Article
    Open Access
    LFA-1 is a leukocyte specific β2 integrin that plays a major role in regulating adhesion and migration of different immune cells. Recent data suggest that LFA-1 on mature dendritic cells (mDCs) may function as a ...