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  • The ER cholesterol sensor SCAP promotes CARTS biogenesis at ER–Golgi membrane contact sites 

    Wakana, Yuichi; Hayashi, Kaito; Nemoto, Takumi; Watanabe, Chiaki; Taoka, Masato; Angulo-Capel, Jessica; Garcia-Parajo, Maria F.; Kumata, Hidetoshi; Umemura, Tomonari; Inoue, Hiroki; Arasaki, Kohei; Campelo, Felix; Tagaya, Mitsuo (Rockefeller University Press, 2020-11-06)
    Article
    Open Access
    In response to cholesterol deprivation, SCAP escorts SREBP transcription factors from the endoplasmic reticulum to the Golgi complex for their proteolytic activation, leading to gene expression for cholesterol synthesis ...

  • Shear forces induce ICAM-1 nanoclustering on endothelial cells that impact on T cell migration 

    Piechocka, Izabela K.; Keary, Sarah; Sosa-Costa, Alberto; Lau, Lukas; Mohan, Nitin; Stanisavljevic, Jelena; Borgman, Kyra J.E.; Lakadamyali, Melike; Manzo, Carlo; Garcia-Parajo, Maria F. (Biophysical Society, 2021-06-01)
    Article
    Open Access
    The leukocyte specific β2-integrin LFA-1, and its ligand ICAM-1 expressed on endothelial cells (ECs), are involved in the arrest, adhesion and transendothelial migration of leukocytes. Although the role of mechanical ...

  • PLANT: A Method for Detecting Changes of Slope in Noisy Trajectories 

    Sosa-Costa, Alberto; Piechocka, Izabela K.; Gardini, Lucia; Pavone, Francesco S.; Capitanio, Marco; Garcia-Parajo, Maria F.; Manzo, Carlo (ScienceDirect, 2018-05-08)
    Article
    Open Access
    Time traces obtained from a variety of biophysical experiments contain valuable information on underlying processes occurring at the molecular level. Accurate quantification of these data can help explain the details of ...

  • Optical Antenna-Based Fluorescence Correlation Spectroscopy to Probe the Nanoscale Dynamics of Biological Membranes 

    Winkler, Pamina M.; Regmi, Raju; Flauraud, Valentin; Brugger, Jürgen; Rigneault, Hervé; Wenger, Jérôme; Garcia-Parajo, Maria F. (ACS, 2017-12-14)
    Article
    Open Access
    The plasma membrane of living cells is compartmentalized at multiple spatial scales ranging from the nano- to the mesoscale. This nonrandom organization is crucial for a large number of cellular functions. At the nanoscale, ...

  • TANGO1 builds a machine for collagen export by recruiting and spatially organizing COPII, tethers and membranes 

    Raote, Ishier; Ortega-Bellido, María; Santos, António J M; Foresti, Ombretta; Zhang, Chong; Garcia-Parajo, Maria F.; Campelo, Felix; Malhotra, Vivek (eLife Sciences Publications, 2018-03-07)
    Article
    Open Access
    Collagen export from the endoplasmic reticulum (ER) requires TANGO1, COPII coats, and retrograde fusion of ERGIC membranes. How do these components come together to produce a transport carrier commensurate with the bulky ...

  • Planar Optical Nanoantennas Resolve Cholesterol-Dependent Nanoscale Heterogeneities in the Plasma Membrane of Living Cells 

    Regmi, Raju; Winkler, Pamina M.; Flauraud, Valentin; Borgman, Kyra J. E.; Manzo, Carlo; Brugger, Jürgen; Rigneault, Hervé; Wenger, Jérôme; Garcia-Parajo, Maria F. (ACS, 2017-09-19)
    Article
    Open Access
    Optical nanoantennas can efficiently confine light into nanoscopic hotspots, enabling single-molecule detection sensitivity at biological relevant conditions. This innovative approach to breach the diffraction limit offers ...

  • Transient Nanoscopic Phase Separation in Biological Lipid Membranes Resolved by Planar Plasmonic Antennas 

    Winkler, Pamina M.; Regmi, Raju; Flauraud, Valentin; Brugger, Jürgen; Rigneault, Hervé; Wenger, Jérôme; Garcia-Parajo, Maria F. (ACS, 2017-07-11)
    Article
    Open Access
    Nanoscale membrane assemblies of sphingolipids, cholesterol, and certain proteins, also known as lipid rafts, play a crucial role in facilitating a broad range of important cell functions. Whereas on living cell membranes ...

  • Sphingolipid metabolic flow controls phosphoinositide turnover at the trans Golgi network 

    Capasso, Serena; Sticco, Lucia; Rizzo, Riccardo; Pirozzi, Marinella; Russo, Domenico; Dathan, Nina A.; Campelo, Felix; Galen, Josse van; Hölttä-Vuori, Maarit; Turacchio, Gabriele; Hausser, Angelika; Malhotra, Vivek; Riezman, Isabelle; Riezman, Howard; Ikonen, Elina; Luberto, Chiara; Parashuraman, Seetharaman; Luini, Alberto; Angelo, Giovanni D (Embo press, 2017-04-10)
    Article
    Open Access
    Sphingolipids are membrane lipids, which are globally required for eukaryotic life. Sphingolipid composition varies among endomembranes with pre- and post-Golgi compartments being poor and rich in sphingolipids, respectively. ...

  • Sphingomyelin metabolism controls the shape and function of the Golgi cisternae 

    Campelo, Felix; Galen, Josse van; Turacchio, Gabriele; Parashuraman, Seetharaman; Kozlov, Michael M.; Garcia-Parajo, Maria F.; Malhotra, Vivek (eLife Sciences Publications, 2017-05-13)
    Article
    Open Access
    The flat Golgi cisterna is a highly conserved feature of eukaryotic cells, but how is this morphology achieved and is it related to its function in cargo sorting and export? A physical model of cisterna morphology led us ...

  • In-plane plasmonic antenna arrays with surface nanogaps for giant fluorescence enhancement 

    Flauraud, Valentin; Regmi, Raju; Winkler, Pamina Martina; Alexander, Duncan T. L.; Rigneault, Herve; Hulst, Niek F. van; Garcia-Parajo, Maria F.; Wenger, Jerome; Brugger, Juergen (ACS, 2017-02-10)
    Article
    Open Access
    Optical nanoantennas have a great potential for enhancing light-matter interactions at the nanometer scale, yet fabrication accuracy and lack of scalability currently limit ultimate antenna performance and applications. ...

  • Roadmap on biosensing and photonics with advanced nano-optical methods 

    Fabrizio, Enzo Di; Schlücker, Sebastian; Wenger, Jérôme; Regmi, Raju; Rigneault, Hervé; Calafiore, Giuseppe; West, Melanie; Cabrini, Stefano; Fleischer, Monika; Hulst, Niek F van; Garcia-Parajo, Maria F.; Pucci, Annemarie; Cojoc, Dan; Hauser, Charlotte A E; Ni, Ming (IOP, 2016-05-10)
    Article
    Open Access
    This roadmap, through the contributions of ten groups worldwide, contains different techniques, methods and materials devoted to sensing in nanomedicine. Optics is used in different ways in the detection schemes. Raman, ...

  • Weak Ergodicity Breaking of Receptor Motion in Living Cells Stemming from Random Diffusivity 

    Manzo, Carlo; Torreno-Pina, Juan A.; Massignan, Pietro; Lapeyre, Gerald J.; Lewenstein, Maciej; Garcia-Parajo, Maria F. (APS Physics, 2015-02-25)
    Article
    Open Access
    Molecular transport in living systems regulates numerous processes underlying biological function. Although many cellular components exhibit anomalous diffusion, only recently has the subdiffusive motion been associated ...

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