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dc.contributor.authorDíaz Blanco, Carlos
dc.contributor.authorOrtner, Andreas
dc.contributor.authorDimitrov, Radostin
dc.contributor.authorNavarro Sentanyes, Antonio
dc.contributor.authorMendoza Gómez, Ernesto
dc.contributor.authorTzanov, Tzanko
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament d'Enginyeria Química
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament de Física i Enginyeria Nuclear
dc.date.accessioned2014-07-28T10:19:41Z
dc.date.created2014-06-23
dc.date.issued2014-06-23
dc.identifier.citationDiaz, C. [et al.]. Building an antifouling zwitterionic coating on urinary catheters using an enzymatically triggered bottom-up approach. "ACS applied materials and interfaces", 23 Juny 2014, vol. 6, núm. 14, p. 11385-11393.
dc.identifier.issn1944-8244
dc.identifier.urihttp://hdl.handle.net/2117/23639
dc.description.abstractCatheter associated urinary tract infections are common during hospitalization due to the formation of bacterial biofilms on the indwelling device. In this study, we report an innovative biotechnology-based approach for the covalent functionalization of silicone catheters with antifouling zwitterionic moieties to prevent biofilm formation. Our approach combines the potential bioactivity of a natural phenolics layer biocatalytically conjugated to sulfobetaine-acrylic residues in an enzymatically initiated surface radical polymerization with laccase. To ensure sufficient coating stability in urine, the silicone catheter is plasma-activated. In contrast to industrial chemical methods, the methacrylate-containing zwitterionic monomers are polymerized at pH 5 and 50 °C using as an initiator the phenoxy radicals solely generated by laccase on the phenolics-coated catheter surface. The coated catheters are characterized by X-ray photoelectron spectroscopy (XPS), Fourier transformed infrared (FTIR) analysis, atomic force microscopy (AFM), and colorimetrically. Contact angle and protein adsorption measurements, coupled with in vitro tests with the Gram-negative Pseudomonas aeruginosa and Gram-positive Staphylococcus aureus in static and dynamic conditions, mimicking the operational conditions to be faced by the catheters, demonstrate reduced biofilm formation by about 80% when compared to that of unmodified urinary catheters. The zwitterionic coating did not affect the viability of the human fibroblasts (BJ-5ta) over seven days, corresponding to the extended useful life of urinary catheters.
dc.format.extent9 p.
dc.language.isoeng
dc.subjectÀrees temàtiques de la UPC::Enginyeria dels materials::Materials plàstics i polímers
dc.subjectÀrees temàtiques de la UPC::Enginyeria química::Biotecnologia
dc.subject.lcshCatheters
dc.subject.lcshPolymerization
dc.subject.lcshBiopolymers
dc.subject.otherEnzymatic polymerization
dc.subject.otherLaccase
dc.subject.otherAntibiofilm Coating
dc.subject.otherUrinary catheter
dc.subject.otherPolydimethylsiloxane
dc.subject.otherArtificial bladder
dc.titleBuilding an antifouling zwitterionic coating on urinary catheters using an enzymatically triggered bottom-up approach
dc.typeArticle
dc.subject.lemacCatèters
dc.subject.lemacPolimerització
dc.subject.lemacBiopolímers
dc.contributor.groupUniversitat Politècnica de Catalunya. SPPT - Superfícies, Productes i Processos Tèxtils
dc.contributor.groupUniversitat Politècnica de Catalunya. GBMI - Grup de Biotecnologia Molecular i Industrial
dc.identifier.doi10.1021/am501961b
dc.description.peerreviewedPeer Reviewed
dc.rights.accessRestricted access - publisher's policy
local.identifier.drac14970256
dc.description.versionPostprint (published version)
dc.relation.projectidinfo:eu-repo/grantAgreement/EC/FP7/278402/EU/Novel approaches for prevention and degeneration of pathogenic bacteria biofilms formed on medical devices e.g. catheters/NOVO
dc.date.lift10000-01-01
local.citation.authorDiaz, C.; Ortner, A.; Dimitrov, R.; Navarro, A.; Mendoza, E.; Tzanov, T.
local.citation.publicationNameACS applied materials and interfaces
local.citation.volume6
local.citation.number14
local.citation.startingPage11385
local.citation.endingPage11393


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