dc.contributor.author | Díaz Blanco, Carlos |
dc.contributor.author | Ortner, Andreas |
dc.contributor.author | Dimitrov, Radostin |
dc.contributor.author | Navarro Sentanyes, Antonio |
dc.contributor.author | Mendoza Gómez, Ernesto |
dc.contributor.author | Tzanov, Tzanko |
dc.contributor.other | Universitat Politècnica de Catalunya. Departament d'Enginyeria Química |
dc.contributor.other | Universitat Politècnica de Catalunya. Departament de Física i Enginyeria Nuclear |
dc.date.accessioned | 2014-07-28T10:19:41Z |
dc.date.created | 2014-06-23 |
dc.date.issued | 2014-06-23 |
dc.identifier.citation | Diaz, C. [et al.]. Building an antifouling zwitterionic coating on urinary catheters using an enzymatically triggered bottom-up approach. "ACS applied materials and interfaces", 23 Juny 2014, vol. 6, núm. 14, p. 11385-11393. |
dc.identifier.issn | 1944-8244 |
dc.identifier.uri | http://hdl.handle.net/2117/23639 |
dc.description.abstract | Catheter associated urinary tract infections are common during hospitalization due to the formation of bacterial biofilms on the indwelling device. In this study, we report an innovative biotechnology-based approach for the covalent functionalization of silicone catheters with antifouling zwitterionic moieties to prevent biofilm formation. Our approach combines the potential bioactivity of a natural phenolics layer biocatalytically conjugated to sulfobetaine-acrylic residues in an enzymatically initiated surface radical polymerization with laccase. To ensure sufficient coating stability in urine, the silicone catheter is plasma-activated. In contrast to industrial chemical methods, the methacrylate-containing zwitterionic monomers are polymerized at pH 5 and 50 °C using as an initiator the phenoxy radicals solely generated by laccase on the phenolics-coated catheter surface. The coated catheters are characterized by X-ray photoelectron spectroscopy (XPS), Fourier transformed infrared (FTIR) analysis, atomic force microscopy (AFM), and colorimetrically. Contact angle and protein adsorption measurements, coupled with in vitro tests with the Gram-negative Pseudomonas aeruginosa and Gram-positive Staphylococcus aureus in static and dynamic conditions, mimicking the operational conditions to be faced by the catheters, demonstrate reduced biofilm formation by about 80% when compared to that of unmodified urinary catheters. The zwitterionic coating did not affect the viability of the human fibroblasts (BJ-5ta) over seven days, corresponding to the extended useful life of urinary catheters. |
dc.format.extent | 9 p. |
dc.language.iso | eng |
dc.subject | Àrees temàtiques de la UPC::Enginyeria dels materials::Materials plàstics i polímers |
dc.subject | Àrees temàtiques de la UPC::Enginyeria química::Biotecnologia |
dc.subject.lcsh | Catheters |
dc.subject.lcsh | Polymerization |
dc.subject.lcsh | Biopolymers |
dc.subject.other | Enzymatic polymerization |
dc.subject.other | Laccase |
dc.subject.other | Antibiofilm Coating |
dc.subject.other | Urinary catheter |
dc.subject.other | Polydimethylsiloxane |
dc.subject.other | Artificial bladder |
dc.title | Building an antifouling zwitterionic coating on urinary catheters using an enzymatically triggered bottom-up approach |
dc.type | Article |
dc.subject.lemac | Catèters |
dc.subject.lemac | Polimerització |
dc.subject.lemac | Biopolímers |
dc.contributor.group | Universitat Politècnica de Catalunya. SPPT - Superfícies, Productes i Processos Tèxtils |
dc.contributor.group | Universitat Politècnica de Catalunya. GBMI - Grup de Biotecnologia Molecular i Industrial |
dc.identifier.doi | 10.1021/am501961b |
dc.description.peerreviewed | Peer Reviewed |
dc.rights.access | Restricted access - publisher's policy |
local.identifier.drac | 14970256 |
dc.description.version | Postprint (published version) |
dc.relation.projectid | info:eu-repo/grantAgreement/EC/FP7/278402/EU/Novel approaches for prevention and degeneration of pathogenic bacteria biofilms formed on medical devices e.g. catheters/NOVO |
dc.date.lift | 10000-01-01 |
local.citation.author | Diaz, C.; Ortner, A.; Dimitrov, R.; Navarro, A.; Mendoza, E.; Tzanov, T. |
local.citation.publicationName | ACS applied materials and interfaces |
local.citation.volume | 6 |
local.citation.number | 14 |
local.citation.startingPage | 11385 |
local.citation.endingPage | 11393 |